Detailed information of TA system TA064653

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/CC2985(antitoxin)
• Location: 580713..581263
• Replicon: plasmid pBSY17_1
• Accession: NZ_CP016453
• Organism: Sphingobium sp. RAC03

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: BSY17_RS02605
• Protein ID: WP_069064223.1
• Coordinates: 580713..581015 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1B3MN42
• Locus tag: BSY17_RS02610
• Protein ID: WP_069064224.1
• Coordinates: 581018..581263 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BSY17_RS02575	576397..576804	+	408	WP_069064219.1	cupin domain-containing protein	-
BSY17_RS02580	577332..577742	-	411	WP_069064492.1	cupin domain-containing protein	-
BSY17_RS02585	577823..578191	-	369	Protein_503	alpha/beta hydrolase	-
BSY17_RS02590	578615..579004	-	390	WP_150125695.1	hypothetical protein	-
BSY17_RS02595	579243..579989	-	747	WP_069064221.1	NAD(P)-binding domain-containing protein	-
BSY17_RS02600	580094..580456	+	363	WP_069064222.1	helix-turn-helix transcriptional regulator	-
BSY17_RS02605	580713..581015	-	303	WP_069064223.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BSY17_RS02610	581018..581263	-	246	WP_069064224.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
BSY17_RS02615	581462..581635	+	174	Protein_509	integrase	-
BSY17_RS02620	581719..582546	-	828	WP_069064226.1	23S rRNA (adenine(2030)-N(6))-methyltransferase RlmJ	-
BSY17_RS02630	582670..583901	+	1232	Protein_511	IS3 family transposase	-
BSY17_RS02635	584226..585032	-	807	WP_069064227.1	S1/P1 nuclease	-
BSY17_RS02640	585111..585443	-	333	WP_043154100.1	polyhydroxyalkanoic acid system family protein	-
BSY17_RS21465	585421..585858	-	438	WP_069064228.1	hypothetical protein	-
BSY17_RS02650	585903..586238	-	336	WP_069064229.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..892708	892708

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-93)

Antitoxin

• Antitoxin ParD (6-64)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11417.96 Da
• Isoelectric Point: 5.1085

>T64653 WP_069064223.1 NZ_CP016453:c581015-580713 [Sphingobium sp. RAC03]
VPRFRLTRAAADDLTTIFLEGIKQFGLPQADTYLEGLSAIFAFLAEYPQAARLREEISPPVRVHPYKAHLVIYDVGDEDQ
VIILRVRHGREDWMSSNYDG

Nucleotide

• Length: 303 bp

>T64653 NZ_CP016453:c581015-580713 [Sphingobium sp. RAC03]
GTGCCTCGCTTTCGCTTAACGCGCGCCGCAGCCGACGATCTGACAACCATCTTTCTCGAAGGCATCAAGCAGTTCGGACT
ACCTCAAGCCGATACCTATCTTGAAGGGCTGAGCGCGATATTCGCGTTTCTTGCCGAGTATCCCCAGGCTGCTCGATTGC
GGGAGGAAATATCGCCTCCCGTCCGCGTACATCCCTACAAGGCACATCTGGTGATTTATGACGTGGGAGATGAGGACCAA
GTCATCATTCTGCGTGTCCGGCATGGCAGGGAGGATTGGATGTCTTCGAACTACGACGGCTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9025.09 Da
• Isoelectric Point: 4.7265

>AT64653 WP_069064224.1 NZ_CP016453:c581263-581018 [Sphingobium sp. RAC03]
MATMNISLPDPMKQWVEAQADTGRYSNASDYVRDLIRRDQERADKVAVVQRLVDEARASGLSDETMADIRTRAISQAGLQ
A

Nucleotide

• Length: 246 bp

>AT64653 NZ_CP016453:c581263-581018 [Sphingobium sp. RAC03]
ATGGCTACGATGAACATCTCACTGCCCGATCCGATGAAGCAATGGGTCGAAGCGCAAGCTGACACTGGTCGGTATAGCAA
TGCCAGTGACTATGTGCGCGATCTCATCCGCCGGGACCAAGAACGCGCCGACAAAGTCGCGGTCGTGCAGCGCCTTGTCG
ATGAGGCCCGCGCCAGCGGTTTGAGCGACGAGACGATGGCGGACATCCGGACGCGAGCGATCAGCCAGGCTGGCTTGCAA
GCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1B3MN42