Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 24408..25078 Replicon plasmid pIEC33019
Accession NZ_CP016446
Organism Pseudomonas putida strain IEC33019

Toxin (Protein)


Gene name vagD Uniprot ID Q2QCR9
Locus tag IEC33019_RS00155 Protein ID WP_012477632.1
Coordinates 24641..25078 (+) Length 146 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q2QCS0
Locus tag IEC33019_RS00150 Protein ID WP_012477631.1
Coordinates 24408..24644 (+) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IEC33019_RS00120 20374..20586 - 213 WP_099592737.1 hypothetical protein -
IEC33019_RS00125 20781..21431 + 651 WP_015272395.1 DNA-binding protein -
IEC33019_RS00135 22199..23620 + 1422 WP_099592740.1 group II intron reverse transcriptase/maturase -
IEC33019_RS00145 23973..24398 + 426 WP_015272397.1 DUF192 domain-containing protein -
IEC33019_RS00150 24408..24644 + 237 WP_012477631.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
IEC33019_RS00155 24641..25078 + 438 WP_012477632.1 type II toxin-antitoxin system VapC family toxin Toxin
IEC33019_RS27445 25085..25246 + 162 WP_015272398.1 hypothetical protein -
IEC33019_RS00160 25374..25634 + 261 WP_015272399.1 hypothetical protein -
IEC33019_RS00165 26315..27151 + 837 WP_015272400.1 DUF945 domain-containing protein -
IEC33019_RS00170 27207..27635 + 429 WP_015272401.1 hypothetical protein -
IEC33019_RS00175 27652..28155 + 504 WP_015272402.1 hypothetical protein -
IEC33019_RS00180 28216..28566 + 351 WP_015272403.1 hypothetical protein -
IEC33019_RS00185 28614..28826 + 213 WP_172959791.1 hypothetical protein -
IEC33019_RS00190 29484..29738 + 255 WP_015272405.1 hypothetical protein -
IEC33019_RS27235 29809..29985 + 177 WP_019692578.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul1 / qacE / aadA11 / qnrVC1 - 1..52710 52710


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 146 a.a.        Molecular weight: 15671.07 Da        Isoelectric Point: 8.5199

>T64642 WP_012477632.1 NZ_CP016446:24641-25078 [Pseudomonas putida]
MSKKTYMLDTNICSFIMRERPDAVLAKLEQAVTNQHRIVVSAITYSEMRFGAANPKASPKVAAMVDAFIQRLDAILSWDA
AAVDQTTQIRTALARLGTPIGNNDAAIAGHALAAGCVLVTNNTREFARVPGLVLEDWTQPAITKS

Download         Length: 438 bp

>T64642 NZ_CP016446:24641-25078 [Pseudomonas putida]
ATGAGCAAAAAGACGTACATGCTCGATACGAACATCTGTTCGTTCATCATGCGCGAGCGCCCCGACGCGGTGCTGGCCAA
GCTGGAACAGGCAGTCACTAATCAGCACCGGATTGTCGTTTCGGCCATCACCTACTCCGAAATGCGTTTCGGGGCCGCCA
ACCCCAAGGCATCGCCCAAGGTTGCAGCGATGGTCGATGCCTTCATCCAGCGCCTGGACGCCATCCTGTCCTGGGACGCC
GCCGCCGTAGATCAAACAACGCAGATCCGCACCGCCCTCGCCCGCCTGGGTACGCCCATTGGCAACAACGATGCAGCGAT
AGCCGGCCATGCCCTGGCCGCTGGGTGTGTTCTGGTCACGAACAACACTCGCGAATTTGCTCGCGTTCCTGGCCTAGTCC
TTGAGGACTGGACCCAACCCGCCATTACCAAGAGTTGA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 9070.29 Da        Isoelectric Point: 4.4563

>AT64642 WP_012477631.1 NZ_CP016446:24408-24644 [Pseudomonas putida]
MRTVSIFMNSRNQAIRIPRDMEYQGVSELEIIRDGETLILRPVRPSWASLRDVAAADPDFLTERKDVIEEDRFLGDFT

Download         Length: 237 bp

>AT64642 NZ_CP016446:24408-24644 [Pseudomonas putida]
ATGCGTACCGTTTCCATTTTCATGAACAGCCGAAACCAGGCGATCCGCATCCCTCGGGATATGGAGTACCAGGGCGTTTC
CGAGCTGGAGATTATCCGCGACGGCGAAACGCTGATCCTGCGCCCTGTGCGCCCCTCCTGGGCATCGTTGCGCGACGTGG
CGGCCGCAGATCCTGACTTTCTGACCGAGCGAAAGGATGTGATCGAGGAAGACCGTTTCCTGGGTGACTTCACATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A010RDD2

Antitoxin

Source ID Structure
AlphaFold DB A0A010SZE6

References