Detailed information of TA system TA064642

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 24408..25078
• Replicon: plasmid pIEC33019
• Accession: NZ_CP016446
• Organism: Pseudomonas putida strain IEC33019

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q2QCR9
• Locus tag: IEC33019_RS00155
• Protein ID: WP_012477632.1
• Coordinates: 24641..25078 (+)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q2QCS0
• Locus tag: IEC33019_RS00150
• Protein ID: WP_012477631.1
• Coordinates: 24408..24644 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IEC33019_RS00120	20374..20586	-	213	WP_099592737.1	hypothetical protein	-
IEC33019_RS00125	20781..21431	+	651	WP_015272395.1	DNA-binding protein	-
IEC33019_RS00135	22199..23620	+	1422	WP_099592740.1	group II intron reverse transcriptase/maturase	-
IEC33019_RS00145	23973..24398	+	426	WP_015272397.1	DUF192 domain-containing protein	-
IEC33019_RS00150	24408..24644	+	237	WP_012477631.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
IEC33019_RS00155	24641..25078	+	438	WP_012477632.1	type II toxin-antitoxin system VapC family toxin	Toxin
IEC33019_RS27445	25085..25246	+	162	WP_015272398.1	hypothetical protein	-
IEC33019_RS00160	25374..25634	+	261	WP_015272399.1	hypothetical protein	-
IEC33019_RS00165	26315..27151	+	837	WP_015272400.1	DUF945 domain-containing protein	-
IEC33019_RS00170	27207..27635	+	429	WP_015272401.1	hypothetical protein	-
IEC33019_RS00175	27652..28155	+	504	WP_015272402.1	hypothetical protein	-
IEC33019_RS00180	28216..28566	+	351	WP_015272403.1	hypothetical protein	-
IEC33019_RS00185	28614..28826	+	213	WP_172959791.1	hypothetical protein	-
IEC33019_RS00190	29484..29738	+	255	WP_015272405.1	hypothetical protein	-
IEC33019_RS27235	29809..29985	+	177	WP_019692578.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul1 / qacE / aadA11 / qnrVC1	-	1..52710	52710

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15671.07 Da
• Isoelectric Point: 8.5199

>T64642 WP_012477632.1 NZ_CP016446:24641-25078 [Pseudomonas putida]
MSKKTYMLDTNICSFIMRERPDAVLAKLEQAVTNQHRIVVSAITYSEMRFGAANPKASPKVAAMVDAFIQRLDAILSWDA
AAVDQTTQIRTALARLGTPIGNNDAAIAGHALAAGCVLVTNNTREFARVPGLVLEDWTQPAITKS

Nucleotide

• Length: 438 bp

>T64642 NZ_CP016446:24641-25078 [Pseudomonas putida]
ATGAGCAAAAAGACGTACATGCTCGATACGAACATCTGTTCGTTCATCATGCGCGAGCGCCCCGACGCGGTGCTGGCCAA
GCTGGAACAGGCAGTCACTAATCAGCACCGGATTGTCGTTTCGGCCATCACCTACTCCGAAATGCGTTTCGGGGCCGCCA
ACCCCAAGGCATCGCCCAAGGTTGCAGCGATGGTCGATGCCTTCATCCAGCGCCTGGACGCCATCCTGTCCTGGGACGCC
GCCGCCGTAGATCAAACAACGCAGATCCGCACCGCCCTCGCCCGCCTGGGTACGCCCATTGGCAACAACGATGCAGCGAT
AGCCGGCCATGCCCTGGCCGCTGGGTGTGTTCTGGTCACGAACAACACTCGCGAATTTGCTCGCGTTCCTGGCCTAGTCC
TTGAGGACTGGACCCAACCCGCCATTACCAAGAGTTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9070.29 Da
• Isoelectric Point: 4.4563

>AT64642 WP_012477631.1 NZ_CP016446:24408-24644 [Pseudomonas putida]
MRTVSIFMNSRNQAIRIPRDMEYQGVSELEIIRDGETLILRPVRPSWASLRDVAAADPDFLTERKDVIEEDRFLGDFT

Nucleotide

• Length: 237 bp

>AT64642 NZ_CP016446:24408-24644 [Pseudomonas putida]
ATGCGTACCGTTTCCATTTTCATGAACAGCCGAAACCAGGCGATCCGCATCCCTCGGGATATGGAGTACCAGGGCGTTTC
CGAGCTGGAGATTATCCGCGACGGCGAAACGCTGATCCTGCGCCCTGTGCGCCCCTCCTGGGCATCGTTGCGCGACGTGG
CGGCCGCAGATCCTGACTTTCTGACCGAGCGAAAGGATGTGATCGAGGAAGACCGTTTCCTGGGTGACTTCACATGA

Structures

Toxin

• AlphaFold DB: A0A010RDD2

Antitoxin

• AlphaFold DB: A0A010SZE6