Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 210297..210874 Replicon chromosome
Accession NZ_CP016443
Organism Burkholderia stabilis strain ATCC BAA-67

Toxin (Protein)


Gene name vapC Uniprot ID A0A1D7ZIN8
Locus tag BBJ41_RS19055 Protein ID WP_069747911.1
Coordinates 210297..210662 (-) Length 122 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1D7ZIN5
Locus tag BBJ41_RS19060 Protein ID WP_069747912.1
Coordinates 210656..210874 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BBJ41_RS19035 206051..206605 + 555 WP_069750264.1 carboxymuconolactone decarboxylase family protein -
BBJ41_RS19040 206756..207481 + 726 WP_069747909.1 extensin -
BBJ41_RS19045 207603..209201 - 1599 WP_069747910.1 alkyl hydroperoxide reductase subunit F -
BBJ41_RS19050 209330..209893 - 564 WP_006488685.1 peroxiredoxin -
BBJ41_RS19055 210297..210662 - 366 WP_069747911.1 type II toxin-antitoxin system VapC family toxin Toxin
BBJ41_RS19060 210656..210874 - 219 WP_069747912.1 CopG family transcriptional regulator Antitoxin
BBJ41_RS19065 210999..211895 - 897 WP_069747913.1 LysR family transcriptional regulator -
BBJ41_RS19070 212073..213239 + 1167 WP_069747914.1 branched-chain amino acid ABC transporter substrate-binding protein -
BBJ41_RS19075 213258..213782 + 525 WP_069747915.1 L-2-amino-thiazoline-4-carboxylic acid hydrolase -
BBJ41_RS19080 213775..215040 + 1266 WP_069747916.1 Zn-dependent hydrolase -
BBJ41_RS19085 215116..215595 - 480 WP_069747917.1 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-106)

Antitoxin

Ribbon-helix-helix protein, CopG

(6-40)


Sequences

Toxin        


Download         Length: 122 a.a.        Molecular weight: 13519.53 Da        Isoelectric Point: 7.0120

>T64632 WP_069747911.1 NZ_CP016443:c210662-210297 [Burkholderia stabilis]
MVKALFDTNILIDYLGGVESARTELARYDYRAISTISWMEVLVGTSTHDEAPIRAWLSSFDVIALDSTIANRAVTIRKER
RIRLPDAIVWASAQVNGLLLVSRNTKDFPAAEPGVRVPYQI

Download         Length: 366 bp

>T64632 NZ_CP016443:c210662-210297 [Burkholderia stabilis]
ATGGTAAAAGCTCTCTTCGACACCAACATCCTGATCGACTATCTCGGCGGTGTGGAATCCGCCCGCACAGAACTTGCCCG
CTATGACTATCGTGCGATCAGCACGATCTCGTGGATGGAGGTCCTGGTCGGTACGTCAACACATGATGAAGCCCCGATCC
GTGCATGGCTTTCGTCGTTCGATGTCATCGCGCTCGACAGCACAATTGCGAATCGCGCCGTGACCATTCGCAAGGAACGC
CGGATACGCTTACCCGACGCGATCGTCTGGGCATCGGCGCAAGTGAACGGGCTGCTGCTCGTGTCGCGCAACACGAAAGA
CTTTCCCGCCGCAGAACCCGGCGTTCGTGTGCCCTATCAAATCTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8304.43 Da        Isoelectric Point: 4.4406

>AT64632 WP_069747912.1 NZ_CP016443:c210874-210656 [Burkholderia stabilis]
MSRILIDLSDGQLDELAVIVETEHRPRAAIIRDAIDAYIALHKRPLADDVFGLWKDRTIDGLAYQEELRSEW

Download         Length: 219 bp

>AT64632 NZ_CP016443:c210874-210656 [Burkholderia stabilis]
TTGAGTCGGATTCTCATTGATCTGTCGGACGGCCAGCTCGACGAGCTGGCCGTCATCGTCGAGACTGAGCATCGTCCCCG
CGCCGCGATCATCCGCGACGCGATCGATGCCTACATTGCCCTGCACAAGCGCCCGCTCGCCGACGACGTCTTCGGTCTCT
GGAAAGATCGCACAATTGACGGGCTCGCCTACCAGGAGGAACTGCGCTCGGAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D7ZIN8

Antitoxin

Source ID Structure
AlphaFold DB A0A1D7ZIN5

References