Detailed information of TA system TA064624

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 1389770..1390365
• Replicon: chromosome
• Accession: NZ_CP016432
• Organism: Prosthecochloris sp. CIB 2401 strain CIB2401

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: Ptc2401_RS06620
• Protein ID: WP_068868242.1
• Coordinates: 1389770..1390075 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A1B1Q2R1
• Locus tag: Ptc2401_RS06625
• Protein ID: WP_068867380.1
• Coordinates: 1390072..1390365 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Ptc2401_RS06585	1384985..1385191	+	207	Protein_1297	putative addiction module antidote protein	-
Ptc2401_RS06590	1385410..1385802	-	393	Protein_1298	SIS domain-containing protein	-
Ptc2401_RS06595	1385987..1386454	-	468	WP_068867376.1	DUF2335 domain-containing protein	-
Ptc2401_RS11240	1386874..1387089	+	216	Protein_1300	DUF4372 domain-containing protein	-
Ptc2401_RS06605	1387151..1388366	-	1216	Protein_1301	IS3 family transposase	-
Ptc2401_RS11430	1388830..1389189	+	360	WP_114616832.1	hypothetical protein	-
Ptc2401_RS06615	1389186..1389389	+	204	WP_068867379.1	hypothetical protein	-
Ptc2401_RS06620	1389770..1390075	+	306	WP_068868242.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
Ptc2401_RS06625	1390072..1390365	+	294	WP_068867380.1	putative addiction module antidote protein	Antitoxin
Ptc2401_RS06630	1390634..1391104	-	471	WP_068867381.1	hypothetical protein	-
Ptc2401_RS06635	1391101..1391550	-	450	WP_068867382.1	helix-turn-helix domain-containing protein	-
Ptc2401_RS06640	1391566..1392132	-	567	WP_068867383.1	hypothetical protein	-
Ptc2401_RS06645	1392411..1392716	+	306	WP_068868243.1	type II toxin-antitoxin system RelE/ParE family toxin	-
Ptc2401_RS06650	1392713..1393006	+	294	WP_068867380.1	putative addiction module antidote protein	-
Ptc2401_RS06655	1393188..1395137	-	1950	WP_068867384.1	glutamine--fructose-6-phosphate transaminase (isomerizing)	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1349007..1398821	49814

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (5-95)

Antitoxin

• Uncharacterized protein HI1420 (6-91)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11472.21 Da
• Isoelectric Point: 10.1931

>T64624 WP_068868242.1 NZ_CP016432:1389770-1390075 [Prosthecochloris sp. CIB 2401]
MNTFIRSSDFDTWLSAMKDQKAKARILCRIQSAIHGNFGDCEPVGEGVSEMRIHVGQGYRLYYTRTGKTVYFLLAGGNKR
TQGKDIVRAKELARKLKETEL

Nucleotide

• Length: 306 bp

>T64624 NZ_CP016432:1389770-1390075 [Prosthecochloris sp. CIB 2401]
ATGAACACCTTCATCCGCTCATCTGATTTCGATACTTGGCTCAGTGCCATGAAAGACCAGAAAGCAAAAGCAAGGATCCT
CTGCCGCATTCAAAGCGCCATACATGGAAACTTTGGCGATTGTGAACCGGTAGGTGAAGGGGTTTCAGAAATGCGGATTC
ACGTGGGGCAAGGATACCGGCTCTATTACACGCGAACAGGAAAAACGGTCTATTTCCTGCTTGCTGGCGGCAACAAGCGA
ACACAGGGAAAAGACATTGTCCGTGCGAAAGAATTAGCCAGAAAACTCAAGGAAACAGAACTATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10321.75 Da
• Isoelectric Point: 7.1993

>AT64624 WP_068867380.1 NZ_CP016432:1390072-1390365 [Prosthecochloris sp. CIB 2401]
MKTTYATFDIAEYLDNDEVIAEYLSVAAADKNPDVFIAAIGDVAKAKGMASIAKTAGLGRESLYKALRKGARPRFDTINA
VLHAIGAKFTVTSADHS

Nucleotide

• Length: 294 bp

>AT64624 NZ_CP016432:1390072-1390365 [Prosthecochloris sp. CIB 2401]
ATGAAAACGACCTACGCAACATTTGACATTGCCGAATACCTCGATAACGATGAGGTCATTGCAGAATACCTTTCTGTTGC
AGCTGCAGACAAAAACCCCGATGTGTTCATTGCTGCTATCGGCGATGTCGCCAAAGCAAAAGGAATGGCCAGCATAGCCA
AGACTGCCGGTCTCGGTCGTGAAAGCCTGTACAAAGCACTGAGAAAAGGTGCCCGGCCGCGCTTTGACACTATCAATGCC
GTCCTGCATGCCATTGGAGCAAAATTCACCGTAACGAGTGCCGATCATTCTTAG

Structures

Antitoxin

• AlphaFold DB: A0A1B1Q2R1