Detailed information of TA system TA064595

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 54107..54629
• Replicon: plasmid pSLy21
• Accession: NZ_CP016405
• Organism: Escherichia coli strain 210221272

Toxin (Protein)

• Gene name: relE
• Uniprot ID: U9YLU1
• Locus tag: BBP24_RS23445
• Protein ID: WP_000638823.1
• Coordinates: 54107..54388 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y7J1
• Locus tag: BBP24_RS23450
• Protein ID: WP_000121741.1
• Coordinates: 54378..54629 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBP24_RS23410	50256..50576	-	321	WP_000362080.1	VirB3 family type IV secretion system protein	-
BBP24_RS25315	50647..50835	-	189	WP_050567133.1	hypothetical protein	-
BBP24_RS23420	50937..51521	-	585	WP_001177113.1	lytic transglycosylase domain-containing protein	-
BBP24_RS23425	51542..51940	-	399	WP_001153665.1	hypothetical protein	-
BBP24_RS23430	52059..52496	-	438	WP_000539665.1	type IV pilus biogenesis protein PilM	-
BBP24_RS23435	52502..53737	-	1236	WP_015059538.1	TcpQ domain-containing protein	-
BBP24_RS23440	53740..54039	-	300	WP_000835764.1	IncN plasmid KikA protein	-
BBP24_RS23445	54107..54388	-	282	WP_000638823.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BBP24_RS23450	54378..54629	-	252	WP_000121741.1	hypothetical protein	Antitoxin
BBP24_RS23455	54729..55364	-	636	WP_015059536.1	hypothetical protein	-
BBP24_RS23460	55412..56244	+	833	Protein_63	DNA primase	-
BBP24_RS23465	56458..56682	-	225	WP_000713561.1	EexN family lipoprotein	-
BBP24_RS23470	56691..57335	-	645	WP_001310442.1	conjugal transfer protein TrbJ	-
BBP24_RS23475	57341..58336	-	996	WP_001028541.1	type IV secretion system protein	-
BBP24_RS23480	58340..58597	-	258	WP_000739144.1	hypothetical protein	-
BBP24_RS25320	58594..58896	-	303	WP_001360345.1	hypothetical protein	-
BBP24_RS23490	59167..59379	-	213	WP_039022940.1	hypothetical protein	-
BBP24_RS25950	59390..59560	-	171	WP_000550720.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mcr-1.1	-	1..63329	63329

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10945.79 Da
• Isoelectric Point: 10.4997

>T64595 WP_000638823.1 NZ_CP016405:c54388-54107 [Escherichia coli]
MIYELAFDHRALKEWQKLGHTVREQFKKKLSERLINPRVPAAKLHGHTDRYKIKLRASGYRLVYQVIDEKVVLLVISVGK
REGSDVYHMADTR

Nucleotide

• Length: 282 bp

>T64595 NZ_CP016405:c54388-54107 [Escherichia coli]
ATGATTTATGAACTGGCATTTGACCACAGAGCACTGAAGGAATGGCAAAAACTCGGTCACACTGTTCGTGAGCAATTCAA
AAAGAAACTGTCAGAACGCCTGATAAATCCACGCGTCCCCGCAGCCAAGCTACATGGTCATACTGACCGTTACAAAATTA
AGCTTCGCGCATCCGGTTACAGGCTCGTTTATCAGGTAATTGATGAGAAGGTCGTTTTGCTTGTGATTTCTGTGGGAAAA
AGAGAAGGCAGCGACGTCTATCACATGGCAGATACCCGCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9223.38 Da
• Isoelectric Point: 3.9823

>AT64595 WP_000121741.1 NZ_CP016405:c54629-54378 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPELYAYYRELAEDAELNAIADERMKNPDIVRVNL
DDL

Nucleotide

• Length: 252 bp

>AT64595 NZ_CP016405:c54629-54378 [Escherichia coli]
ATGAGCTACCAAATTCTGACTACGACAGCAGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACTGTAGCCGAAGG
AGAAGGGGATGCCGTCGCAATCCTGAACCGAAATGAACCAGCGTTCTACTGTGTCCCACCAGAACTTTATGCCTACTACC
GGGAACTCGCTGAAGACGCTGAACTCAACGCAATTGCTGATGAAAGAATGAAAAATCCAGACATTGTAAGGGTTAACCTG
GATGATTTATGA

Structures

Toxin

• AlphaFold DB: U9YLU1

Antitoxin

• AlphaFold DB: U9Y7J1