Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3798093..3798747 Replicon chromosome
Accession NZ_CP016404
Organism Escherichia coli strain 210221272

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag BBP24_RS18750 Protein ID WP_000244777.1
Coordinates 3798093..3798500 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag BBP24_RS18755 Protein ID WP_000354046.1
Coordinates 3798481..3798747 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BBP24_RS18730 3794050..3795783 - 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -
BBP24_RS18735 3795789..3796499 - 711 WP_069684501.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
BBP24_RS18740 3796524..3797420 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
BBP24_RS18745 3797532..3798053 + 522 WP_001055874.1 flavodoxin FldB -
BBP24_RS18750 3798093..3798500 - 408 WP_000244777.1 toxin CptA Toxin
BBP24_RS18755 3798481..3798747 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
BBP24_RS18760 3798990..3799970 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
BBP24_RS18765 3800166..3800825 - 660 WP_000250274.1 hemolysin III family protein -
BBP24_RS18770 3800989..3801300 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
BBP24_RS18775 3801345..3802778 + 1434 WP_069684555.1 6-phospho-beta-glucosidase BglA -
BBP24_RS18780 3802835..3803578 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T64590 WP_000244777.1 NZ_CP016404:c3798500-3798093 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T64590 NZ_CP016404:c3798500-3798093 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT64590 WP_000354046.1 NZ_CP016404:c3798747-3798481 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT64590 NZ_CP016404:c3798747-3798481 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References