Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1204305..1204923 Replicon chromosome
Accession NZ_CP016404
Organism Escherichia coli strain 210221272

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag BBP24_RS05970 Protein ID WP_001291435.1
Coordinates 1204305..1204523 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag BBP24_RS05975 Protein ID WP_000344800.1
Coordinates 1204549..1204923 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BBP24_RS05935 1199594..1200166 + 573 WP_000779831.1 YbaY family lipoprotein -
BBP24_RS05940 1200197..1200508 - 312 WP_000409911.1 MGMT family protein -
BBP24_RS05950 1200887..1201240 + 354 WP_000878140.1 DUF1428 domain-containing protein -
BBP24_RS05955 1201282..1202832 - 1551 WP_032265872.1 cyclic-guanylate-specific phosphodiesterase PdeB -
BBP24_RS05960 1202996..1203466 - 471 WP_000136192.1 YlaC family protein -
BBP24_RS05965 1203582..1204133 - 552 WP_000102564.1 maltose O-acetyltransferase -
BBP24_RS05970 1204305..1204523 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
BBP24_RS05975 1204549..1204923 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
BBP24_RS05980 1205469..1208618 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
BBP24_RS05985 1208641..1209834 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T64577 WP_001291435.1 NZ_CP016404:c1204523-1204305 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T64577 NZ_CP016404:c1204523-1204305 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT64577 WP_000344800.1 NZ_CP016404:c1204923-1204549 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT64577 NZ_CP016404:c1204923-1204549 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References