Detailed information of TA system TA064573

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 468934..469532
• Replicon: chromosome
• Accession: NZ_CP016404
• Organism: Escherichia coli strain 210221272

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A8T6PRV3
• Locus tag: BBP24_RS02275
• Protein ID: WP_053291281.1
• Coordinates: 469155..469532 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: L4J1C0
• Locus tag: BBP24_RS02270
• Protein ID: WP_001603498.1
• Coordinates: 468934..469155 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBP24_RS02255	464534..465214	+	681	WP_053270475.1	fimbrial assembly chaperone	-
BBP24_RS02260	465232..467718	+	2487	WP_069684252.1	fimbria/pilus outer membrane usher protein	-
BBP24_RS02265	467730..468749	+	1020	WP_069684253.1	type 1 fimbrial protein	-
BBP24_RS02270	468934..469155	+	222	WP_001603498.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
BBP24_RS02275	469155..469532	+	378	WP_053291281.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
BBP24_RS25420	469642..469857	-	216	Protein_404	transposase	-
BBP24_RS02285	470043..471305	-	1263	WP_069684254.1	integrase arm-type DNA-binding domain-containing protein	-
BBP24_RS02295	471685..472260	-	576	WP_001188520.1	transcriptional regulator	-
BBP24_RS02300	472297..473994	-	1698	WP_000068902.1	protein-disulfide reductase DsbD	-
BBP24_RS02305	473970..474308	-	339	WP_000883400.1	divalent cation tolerance protein CutA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	htpB	443832..483176	39344
	-	flank	IS/Tn	-	-	469576..469857	281

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13487.28 Da
• Isoelectric Point: 6.2257

>T64573 WP_053291281.1 NZ_CP016404:469155-469532 [Escherichia coli]
MRHISPEELIAIHDANISRYGGLPGMPDSGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVYDSPELAELTVGAATGEVSVSTVVATLRRLYGTA

Nucleotide

• Length: 378 bp

>T64573 NZ_CP016404:469155-469532 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAGCTTATTGCAATTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCAGGCATGCC
AGATTCGGGAAGAGCTGAGGCTATTATCGGGAGAGTCCAGGCCAGAGTTGCATATGAAGAGATTACCGACCTTTTCGAAG
TCTCCGCAACTTACCTGGTGGCTACTGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
TTGTTATTTCTGCGCCGTAACGGTGTGCAGGTATATGATTCACCAGAACTGGCAGAGCTTACTGTGGGGGCTGCGACCGG
AGAGGTATCCGTATCTACTGTCGTTGCCACGTTACGTAGATTGTATGGTACGGCGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8190.24 Da
• Isoelectric Point: 6.3159

>AT64573 WP_001603498.1 NZ_CP016404:468934-469155 [Escherichia coli]
MQSINFRTARSNLTEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDTTNKKLVNR

Nucleotide

• Length: 222 bp

>AT64573 NZ_CP016404:468934-469155 [Escherichia coli]
ATGCAATCTATTAACTTCCGTACCGCACGCAGCAATCTTACCGAAGTGCTCAACAATGTTGAAGCCGGAGAAGAGGTTGA
AATCACCCGTAGAGGCCGTGAGCCAGCTGTAATTGTCAGCAAGGCCACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTCGCATCCCTGTTTGACACTCTGGACACCACCAATAAGAAACTGGTTAACCGATAA

Structures

Antitoxin

• AlphaFold DB: L4J1C0