Detailed information of TA system TA064572

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 438693..439528
• Replicon: chromosome
• Accession: NZ_CP016404
• Organism: Escherichia coli strain 210221272

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7W4PV54
• Locus tag: BBP24_RS02090
• Protein ID: WP_000854821.1
• Coordinates: 438693..439070 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7MK11
• Locus tag: BBP24_RS02095
• Protein ID: WP_001285610.1
• Coordinates: 439160..439528 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBP24_RS02065	435060..436598	-	1539	WP_001187177.1	lysine decarboxylation/transport transcriptional activator CadC	-
BBP24_RS02075	437347..438189	-	843	WP_001280501.1	DUF4942 domain-containing protein	-
BBP24_RS02080	438274..438471	-	198	WP_023563519.1	DUF957 domain-containing protein	-
BBP24_RS25400	438547..438696	-	150	Protein_369	hypothetical protein	-
BBP24_RS02090	438693..439070	-	378	WP_000854821.1	type IV toxin-antitoxin system toxin CbtA	Toxin
BBP24_RS02095	439160..439528	-	369	WP_001285610.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
BBP24_RS02100	439608..439829	-	222	WP_000692350.1	DUF987 domain-containing protein	-
BBP24_RS02105	439916..440392	-	477	WP_063091579.1	RadC family protein	-
BBP24_RS02110	440408..440887	-	480	WP_000860074.1	antirestriction protein	-
BBP24_RS02115	440969..441787	-	819	WP_001234688.1	DUF945 domain-containing protein	-
BBP24_RS25405	441888..442040	-	153	WP_001696589.1	DUF905 family protein	-
BBP24_RS02125	442046..442723	-	678	WP_063091580.1	hypothetical protein	-
BBP24_RS02130	442842..443726	-	885	WP_023563932.1	50S ribosome-binding GTPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13930.95 Da
• Isoelectric Point: 8.5163

>T64572 WP_000854821.1 NZ_CP016404:c439070-438693 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T64572 NZ_CP016404:c439070-438693 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13606.39 Da
• Isoelectric Point: 6.0618

>AT64572 WP_001285610.1 NZ_CP016404:c439528-439160 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT64572 NZ_CP016404:c439528-439160 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAATTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7W4PV54