Detailed information of TA system TA064563

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2086021..2086550
• Replicon: chromosome
• Accession: NZ_CP016398
• Organism: Staphylococcus aureus strain FORC_040

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: FORC40_RS10680
• Protein ID: WP_000621175.1
• Coordinates: 2086021..2086383 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: FORC40_RS10685
• Protein ID: WP_000948331.1
• Coordinates: 2086380..2086550 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC40_RS10655	2083000..2083770	-	771	WP_088867610.1	RNA polymerase sigma factor SigB	-
FORC40_RS10660	2083745..2084224	-	480	WP_088867611.1	anti-sigma B factor RsbW	-
FORC40_RS10665	2084226..2084552	-	327	WP_001052491.1	anti-sigma factor antagonist	-
FORC40_RS10670	2084671..2085672	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
FORC40_RS10680	2086021..2086383	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FORC40_RS10685	2086380..2086550	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
FORC40_RS10690	2086635..2087783	-	1149	WP_001281142.1	alanine racemase	-
FORC40_RS10695	2087849..2088208	-	360	WP_000581197.1	holo-ACP synthase	-
FORC40_RS10700	2088212..2088703	-	492	WP_001205910.1	PH domain-containing protein	-
FORC40_RS10705	2088690..2090273	-	1584	WP_001294626.1	PH domain-containing protein	-
FORC40_RS10710	2090266..2090746	-	481	Protein_1976	hypothetical protein	-
FORC40_RS10715	2090955..2091515	-	561	WP_001092402.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T64563 WP_000621175.1 NZ_CP016398:c2086383-2086021 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T64563 NZ_CP016398:c2086383-2086021 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT64563 WP_000948331.1 NZ_CP016398:c2086550-2086380 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT64563 NZ_CP016398:c2086550-2086380 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1