Detailed information of TA system TA064551

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 3028152..3028805
• Replicon: chromosome
• Accession: NZ_CP016396
• Organism: Mycobacterium avium strain RCAD0278 isolate SC150824

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: BBJ32_RS14170
• Protein ID: WP_009975163.1
• Coordinates: 3028344..3028805 (+)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: Q742J1
• Locus tag: BBJ32_RS14165
• Protein ID: WP_003872881.1
• Coordinates: 3028152..3028340 (+)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBJ32_RS14155	3024135..3025721	+	1587	WP_009975161.1	serine hydrolase	-
BBJ32_RS14160	3025718..3028090	+	2373	WP_009975162.1	cation-translocating P-type ATPase	-
BBJ32_RS14165	3028152..3028340	+	189	WP_003872881.1	antitoxin	Antitoxin
BBJ32_RS14170	3028344..3028805	+	462	WP_009975163.1	SRPBCC family protein	Toxin
BBJ32_RS14175	3028874..3029308	+	435	WP_023880080.1	hypothetical protein	-
BBJ32_RS14180	3030304..3031887	+	1584	WP_003872878.1	DUF4185 domain-containing protein	-
BBJ32_RS14185	3031927..3032313	+	387	WP_009975165.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (2-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-51)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 16627.13 Da
• Isoelectric Point: 9.5654

>T64551 WP_009975163.1 NZ_CP016396:3028344-3028805 [Mycobacterium avium]
VAKLSGSIDVPLPPEVAWQHASDLSRYKEWLTIHRVWRSTLPDQNDKGTVVESIVEVKGMLNRIKWTVVRYKPPEGMTLN
GDGVGGVKVKLMAKVQPKADGSVVSFDVHLGGPALFGPIGMIVAAALRGDIDQSLENFVTVFARSDPSTNGHR

Nucleotide

• Length: 462 bp

>T64551 NZ_CP016396:3028344-3028805 [Mycobacterium avium]
GTGGCCAAGCTATCCGGATCCATCGATGTCCCGCTGCCCCCCGAGGTGGCCTGGCAGCACGCCTCCGACCTGTCCCGGTA
CAAGGAGTGGCTGACCATTCACCGGGTGTGGCGCAGCACCTTGCCCGACCAGAACGACAAGGGCACCGTCGTCGAGTCGA
TCGTCGAGGTCAAGGGCATGCTCAACCGGATCAAATGGACGGTGGTGCGCTACAAGCCACCGGAGGGCATGACGCTCAAC
GGCGACGGCGTCGGCGGGGTCAAGGTCAAGCTGATGGCCAAGGTGCAGCCCAAGGCGGACGGTTCCGTCGTCAGCTTCGA
CGTGCACCTCGGCGGTCCGGCGCTGTTCGGGCCCATCGGCATGATCGTCGCGGCCGCGCTGCGCGGCGACATCGACCAGT
CGCTGGAGAACTTCGTCACGGTGTTCGCCCGATCGGATCCGAGCACCAACGGTCATCGCTGA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6745.42 Da
• Isoelectric Point: 4.4865

>AT64551 WP_003872881.1 NZ_CP016396:3028152-3028340 [Mycobacterium avium]
MGFLDKAKDLLSQNADKVEMAIDKAGEFVDEKTQGKYSDTIHQVQEQAKKATGATETGDQQG

Nucleotide

• Length: 189 bp

>AT64551 NZ_CP016396:3028152-3028340 [Mycobacterium avium]
ATGGGATTCCTGGACAAGGCCAAGGACCTGTTGTCGCAGAACGCCGACAAGGTCGAGATGGCCATCGACAAGGCCGGCGA
GTTCGTCGACGAGAAGACGCAGGGCAAGTACTCCGACACCATCCACCAGGTGCAGGAGCAGGCCAAGAAGGCCACCGGCG
CCACCGAGACCGGCGACCAGCAGGGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E2WC18