Detailed information of TA system TA064550

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 665959..666523
• Replicon: chromosome
• Accession: NZ_CP016396
• Organism: Mycobacterium avium strain RCAD0278 isolate SC150824

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: X7VBD8
• Locus tag: BBJ32_RS02830
• Protein ID: WP_003875391.1
• Coordinates: 665959..666330 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: BBJ32_RS22815
• Protein ID: WP_011724021.1
• Coordinates: 666317..666523 (-)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBJ32_RS02795	661125..661448	-	324	WP_009975396.1	antibiotic biosynthesis monooxygenase	-
BBJ32_RS02800	661860..662383	-	524	Protein_555	peptidoglycan endopeptidase	-
BBJ32_RS02805	662540..662893	-	354	WP_003875396.1	hypothetical protein	-
BBJ32_RS22810	662963..663346	-	384	WP_023884114.1	hypothetical protein	-
BBJ32_RS02810	663415..663813	-	399	WP_023865349.1	VOC family protein	-
BBJ32_RS02815	663915..664520	-	606	WP_009975394.1	TetR/AcrR family transcriptional regulator; helix-turn-helix transcriptional regulator	-
BBJ32_RS02820	664616..665353	+	738	WP_009975393.1	SDR family oxidoreductase	-
BBJ32_RS02825	665419..665859	-	441	WP_023884113.1	nitroreductase family deazaflavin-dependent oxidoreductase	-
BBJ32_RS02830	665959..666330	-	372	WP_003875391.1	type II toxin-antitoxin system VapC family toxin	Toxin
BBJ32_RS22815	666317..666523	-	207	WP_011724021.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
BBJ32_RS02835	666692..667636	-	945	WP_023884112.1	HNH endonuclease	-
BBJ32_RS02840	667836..668909	-	1074	WP_009975391.1	redox-regulated ATPase YchF	-
BBJ32_RS02845	669040..670233	+	1194	WP_080516334.1	hypothetical protein	-
BBJ32_RS02850	670250..671248	-	999	WP_009975388.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-112)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13770.14 Da
• Isoelectric Point: 6.6003

>T64550 WP_003875391.1 NZ_CP016396:c666330-665959 [Mycobacterium avium]
MILVDTSVWIDHLHVADKRLIEFLTNDAIGCHQMVIEELALGAIRRRADLLRLLSNLRAFPLLTHAEVLHLVECHRLWGR
GLSAIDVHLLGSVALVDGARLWTRDKNLKAAGWDVGIAIVDEL

Nucleotide

• Length: 372 bp

>T64550 NZ_CP016396:c666330-665959 [Mycobacterium avium]
ATGATTCTCGTTGACACGTCGGTATGGATCGATCATCTGCACGTCGCCGACAAGCGACTCATAGAGTTCCTGACCAACGA
CGCAATCGGTTGCCACCAAATGGTGATCGAGGAGTTGGCATTGGGTGCGATCAGGCGGCGCGCCGATCTATTGCGCCTGC
TGTCCAATCTTCGGGCGTTCCCGTTGCTGACGCATGCGGAGGTCCTTCATCTCGTCGAATGCCACCGCTTGTGGGGCCGC
GGACTGAGTGCGATCGACGTCCATCTTCTCGGGTCGGTTGCTCTGGTCGACGGTGCACGGCTATGGACCAGGGACAAGAA
TTTAAAAGCGGCGGGCTGGGACGTGGGTATCGCGATCGTCGACGAGCTCTGA

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 7261.25 Da
• Isoelectric Point: 11.0634

>AT64550 WP_011724021.1 NZ_CP016396:c666523-666317 [Mycobacterium avium]
MRTTVTIDDALLAEAAELTGVTESVALLRQGLQTLVRVESARRLAALGGTDRKASVGPRRRTPADDSR

Nucleotide

• Length: 207 bp

>AT64550 NZ_CP016396:c666523-666317 [Mycobacterium avium]
ATGAGGACGACGGTGACGATTGACGACGCCTTATTGGCAGAGGCGGCCGAACTGACCGGGGTGACAGAGAGCGTGGCGCT
CCTCCGTCAGGGACTGCAGACACTCGTTCGGGTAGAGAGCGCCCGTCGGCTGGCGGCGCTCGGCGGCACGGATCGGAAGG
CATCGGTCGGCCCTCGCCGGAGGACACCCGCCGATGATTCTCGTTGA

Structures

Toxin

• AlphaFold DB: A0A0E2WB37