Detailed information of TA system TA064536

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemK-mazE/MazF(toxin)
• Location: 77260..77887
• Replicon: chromosome
• Accession: NZ_CP016379
• Organism: Anoxybacter fermentans strain DY22613

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: BBF96_RS00310
• Protein ID: WP_127015313.1
• Coordinates: 77537..77887 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: BBF96_RS00305
• Protein ID: WP_127015312.1
• Coordinates: 77260..77535 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBF96_RS00290	72465..73607	+	1143	WP_205665669.1	alanine racemase	-
BBF96_RS00295	73680..74453	+	774	WP_127015310.1	alpha/beta fold hydrolase	-
BBF96_RS00300	74585..76768	+	2184	WP_127015311.1	DbpA RNA binding domain-containing protein	-
BBF96_RS16295	76891..77040	+	150	WP_164730814.1	hypothetical protein	-
BBF96_RS00305	77260..77535	+	276	WP_127015312.1	ribbon-helix-helix protein, CopG family	Antitoxin
BBF96_RS00310	77537..77887	+	351	WP_127015313.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BBF96_RS00315	78010..79011	+	1002	WP_127015314.1	asparaginase	-
BBF96_RS00320	79032..80210	+	1179	WP_127015315.1	amidohydrolase	-
BBF96_RS00325	80239..82596	+	2358	WP_127015316.1	DUF4292 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-43)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12936.92 Da
• Isoelectric Point: 5.7528

>T64536 WP_127015313.1 NZ_CP016379:77537-77887 [Anoxybacter fermentans]
VSIKRGDVYYADLNPVVGSEQGGVRPVLVIQNDIGNKYSPTVIVAAITSKIDKAKLPTHVEIKAEQSNLEKDSVILLEQI
RTIDKRRLQRQVTHLDPEIIERVNEALEISLGLIEL

Nucleotide

• Length: 351 bp

>T64536 NZ_CP016379:77537-77887 [Anoxybacter fermentans]
GTGTCTATAAAACGTGGTGATGTATATTATGCTGATTTGAATCCGGTTGTAGGGTCTGAGCAGGGTGGTGTACGGCCAGT
TCTGGTTATTCAAAATGATATTGGTAATAAATATAGCCCAACTGTAATAGTTGCTGCTATTACTTCTAAAATTGATAAAG
CTAAATTACCAACACATGTTGAGATAAAAGCTGAGCAAAGTAATCTTGAGAAGGATTCTGTAATTTTATTAGAACAGATC
CGGACTATCGATAAGCGTAGATTACAGCGGCAGGTGACTCATCTAGATCCGGAGATTATTGAGCGGGTAAATGAAGCTTT
AGAAATTAGTCTCGGGCTAATTGAACTTTAA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10444.19 Da
• Isoelectric Point: 5.8799

>AT64536 WP_127015312.1 NZ_CP016379:77260-77535 [Anoxybacter fermentans]
MGNLKRVMISLPSSLLKEVDGIVKKEHGNRSQFIREAMQMYIAERQKKELREKMKQGYIEMGQINLELANAGFSVDCGMM
LAYEENLAECE

Nucleotide

• Length: 276 bp

>AT64536 NZ_CP016379:77260-77535 [Anoxybacter fermentans]
TTGGGTAACTTAAAACGTGTTATGATTAGCTTGCCGAGCAGCCTTCTTAAAGAAGTGGATGGAATTGTAAAAAAAGAACA
TGGAAATCGGAGTCAATTTATACGGGAAGCTATGCAAATGTATATAGCAGAACGTCAAAAAAAGGAACTTCGTGAAAAAA
TGAAACAGGGATATATAGAGATGGGTCAGATTAATCTGGAATTGGCCAACGCTGGTTTTTCTGTTGATTGTGGAATGATG
CTAGCATATGAGGAAAATCTTGCGGAGTGTGAGTAA