Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 490046..490683 Replicon chromosome
Accession NZ_CP016371
Organism Bacillus velezensis strain S3-1

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag A5891_RS02485 Protein ID WP_003156187.1
Coordinates 490333..490683 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag A5891_RS02480 Protein ID WP_003156188.1
Coordinates 490046..490327 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A5891_RS02460 486411..487010 - 600 WP_032872997.1 rhomboid family intramembrane serine protease -
A5891_RS02465 487103..487468 + 366 WP_007609580.1 holo-ACP synthase -
A5891_RS02470 487633..488640 + 1008 WP_032872999.1 outer membrane lipoprotein carrier protein LolA -
A5891_RS02475 488757..489926 + 1170 WP_032873001.1 alanine racemase -
A5891_RS02480 490046..490327 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
A5891_RS02485 490333..490683 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
A5891_RS02490 490801..491622 + 822 WP_003156182.1 STAS domain-containing protein -
A5891_RS02495 491627..491992 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
A5891_RS02500 491995..492396 + 402 WP_003156178.1 anti-sigma regulatory factor -
A5891_RS02505 492408..493415 + 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
A5891_RS02510 493479..493808 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
A5891_RS02515 493805..494287 + 483 WP_007609591.1 anti-sigma B factor RsbW -
A5891_RS02520 494253..495041 + 789 WP_032873003.1 RNA polymerase sigma factor SigB -
A5891_RS02525 495041..495643 + 603 WP_007410234.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T64532 WP_003156187.1 NZ_CP016371:490333-490683 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T64532 NZ_CP016371:490333-490683 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT64532 WP_003156188.1 NZ_CP016371:490046-490327 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT64532 NZ_CP016371:490046-490327 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References