Detailed information of TA system TA064531

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2388651..2389629
• Replicon: chromosome
• Accession: NZ_CP016360
• Organism: Bacillus cereus strain M13

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: W8Y388
• Locus tag: BA201_RS12810
• Protein ID: WP_000624977.1
• Coordinates: 2388651..2389388 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: M1PKC2
• Locus tag: BA201_RS12815
• Protein ID: WP_000237819.1
• Coordinates: 2389501..2389629 (+)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BA201_RS12780	2384264..2384671	+	408	WP_000063587.1	VOC family protein	-
BA201_RS12785	2384695..2385075	+	381	WP_002025805.1	hypothetical protein	-
BA201_RS12790	2385233..2386918	-	1686	WP_002025806.1	alpha-keto acid decarboxylase family protein	-
BA201_RS12795	2387026..2387508	+	483	WP_000191888.1	MarR family winged helix-turn-helix transcriptional regulator	-
BA201_RS12800	2387675..2388412	+	738	WP_000594152.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
BA201_RS12810	2388651..2389388	+	738	WP_000624977.1	stage II sporulation protein SA	Toxin
BA201_RS12815	2389501..2389629	+	129	WP_000237819.1	hypothetical protein	Antitoxin
BA201_RS12820	2389702..2389878	+	177	WP_000852629.1	stage II sporulation protein SB	-
BA201_RS12825	2389898..2390287	-	390	WP_000713862.1	YxeA family protein	-
BA201_RS12830	2390499..2391968	+	1470	WP_000287555.1	beta-Ala-His dipeptidase	-
BA201_RS12835	2392214..2393023	+	810	WP_000678368.1	C39 family peptidase	-
BA201_RS12840	2393049..2393651	+	603	WP_000517274.1	hypothetical protein	-
BA201_RS12845	2393895..2394146	-	252	WP_000827560.1	response regulator transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28307.03 Da
• Isoelectric Point: 8.2998

>T64531 WP_000624977.1 NZ_CP016360:2388651-2389388 [Bacillus cereus]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T64531 NZ_CP016360:2388651-2389388 [Bacillus cereus]
ATGATCTCTAACATTCGAATCGGCTTATTTGTTTTAGCGATTGTCTTTGTAGTCCTTGTTTTCTTTTATTGGAAAAATGA
GGAGTTGTACGAGGAGAAAAAGCAACGTATTAGAAAGACTTGGTATGGTTTGTTTATCATATCTGTCACCGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCAATGGTTATTTTTGTTGATATTGCGTTC
ATTTTAACGCCTAATATTTCAGAAATATGGGGTGCGAAATTTAGCGATATTGGCAAGACAGTTCAATCAATAAAACGATC
ATTAATTGCATCTAAAGCGAGAGGGGAAATATACACGACAATTATTCAAAATGTTAATCCAGCAGTATTCGGTACGATGG
AATGGCATACGGAAGAGGAATATACAAAGAGCTTAAATGCATTTTTAGATTCATATGGGGAAAAGATTGGCGCGAAAATT
GTTGTTTTTGAAGCCGCGAAAGAGTTAAATACAAACTTTCGTGGTATTCGCTCGCAATTTAGTATTATCGTTCCGTTAGA
ACATATCGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGGATTATACCAGCAAAAATAGTAAGTGACG
TTTTCATTGTTATTGATGGAAAGAAAAATAACCTGCAAGATCGGGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 4953.36 Da
• Isoelectric Point: 3.9798

>AT64531 WP_000237819.1 NZ_CP016360:2389501-2389629 [Bacillus cereus]
VVKDYDNDFFKEGNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT64531 NZ_CP016360:2389501-2389629 [Bacillus cereus]
GTGGTAAAAGATTATGACAATGATTTTTTTAAAGAAGGTAACGAAGATACAACAGATTTCTCTTTAATAAAAGGGGATAC
GTTGCAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATCATAA

Structures

Toxin

• AlphaFold DB: W8Y388

Antitoxin

• AlphaFold DB: M1PKC2