Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 239955..240597 Replicon chromosome
Accession NZ_CP016360
Organism Bacillus cereus strain M13

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag BA201_RS01395 Protein ID WP_000635963.1
Coordinates 240247..240597 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag BA201_RS01390 Protein ID WP_000004570.1
Coordinates 239955..240242 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BA201_RS01365 235272..236234 + 963 WP_000961165.1 UV DNA damage repair endonuclease UvsE -
BA201_RS01370 236227..236799 - 573 WP_000908523.1 rhomboid family intramembrane serine protease -
BA201_RS01375 236893..237252 + 360 WP_000583416.1 holo-ACP synthase -
BA201_RS01380 237409..238359 + 951 WP_002004297.1 outer membrane lipoprotein carrier protein LolA -
BA201_RS01385 238477..239646 + 1170 WP_000390615.1 alanine racemase -
BA201_RS01390 239955..240242 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
BA201_RS01395 240247..240597 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BA201_RS01400 240665..242833 + 2169 WP_000426245.1 RNA-binding transcriptional accessory protein -
BA201_RS01405 242891..243007 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
BA201_RS01410 243203..243661 + 459 WP_000344233.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T64530 WP_000635963.1 NZ_CP016360:240247-240597 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T64530 NZ_CP016360:240247-240597 [Bacillus cereus]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATAACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT64530 WP_000004570.1 NZ_CP016360:239955-240242 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT64530 NZ_CP016360:239955-240242 [Bacillus cereus]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References