Detailed information of TA system TA064510

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1748714..1749548
• Replicon: chromosome
• Accession: NZ_CP016358
• Organism: Escherichia coli strain K-15KW01

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A838AQY5
• Locus tag: BA058_RS08430
• Protein ID: WP_023152077.1
• Coordinates: 1749171..1749548 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: BA058_RS08425
• Protein ID: WP_065377585.1
• Coordinates: 1748714..1749082 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BA058_RS08390	1745529..1745984	+	456	WP_000581504.1	hypothetical protein	-
BA058_RS08395	1746063..1746296	+	234	WP_001119729.1	DUF905 family protein	-
BA058_RS08400	1746396..1747214	+	819	WP_032250973.1	DUF945 domain-containing protein	-
BA058_RS08405	1747296..1747775	+	480	WP_023568406.1	antirestriction protein	-
BA058_RS08410	1747791..1748267	+	477	WP_001186773.1	RadC family protein	-
BA058_RS08415	1748330..1748551	+	222	WP_000692305.1	DUF987 domain-containing protein	-
BA058_RS08425	1748714..1749082	+	369	WP_065377585.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
BA058_RS08430	1749171..1749548	+	378	WP_023152077.1	type IV toxin-antitoxin system toxin CbtA	Toxin
BA058_RS08435	1749545..1750033	+	489	WP_065377586.1	hypothetical protein	-
BA058_RS08440	1750045..1750242	+	198	WP_000839281.1	DUF957 domain-containing protein	-
BA058_RS08445	1750339..1750848	+	510	WP_050582268.1	DUF4942 domain-containing protein	-
BA058_RS08450	1751251..1751421	+	171	Protein_1612	IS110 family transposase	-
BA058_RS08455	1752220..1753203	+	984	WP_001331698.1	KpsF/GutQ family sugar-phosphate isomerase	-
BA058_RS08460	1753275..1754423	+	1149	WP_000905922.1	capsule polysaccharide export inner-membrane protein KpsE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1751317..1751421	104

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14056.12 Da
• Isoelectric Point: 7.8839

>T64510 WP_023152077.1 NZ_CP016358:1749171-1749548 [Escherichia coli]
MKTLPVLPGQVASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRAIGLMTRDNYRTVNNITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T64510 NZ_CP016358:1749171-1749548 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGTGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCATTACGGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATTCTCCGGGCTCGCAGGGCAATCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCAGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13580.37 Da
• Isoelectric Point: 5.0580

>AT64510 WP_065377585.1 NZ_CP016358:1748714-1749082 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHPDQAFPLLMKQPELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT64510 NZ_CP016358:1748714-1749082 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCGGATGCGTACCATCCGGACCAGGCCTTTCCGCTGCTGATGAAACAACCGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A838AQY5