Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1585983..1586637 Replicon chromosome
Accession NZ_CP016358
Organism Escherichia coli strain K-15KW01

Toxin (Protein)


Gene name cptA Uniprot ID F4T2L4
Locus tag BA058_RS07555 Protein ID WP_000244765.1
Coordinates 1585983..1586390 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag BA058_RS07560 Protein ID WP_000354046.1
Coordinates 1586371..1586637 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BA058_RS07535 1581940..1583673 - 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -
BA058_RS07540 1583679..1584389 - 711 WP_000715229.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
BA058_RS07545 1584414..1585310 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
BA058_RS07550 1585422..1585943 + 522 WP_001055874.1 flavodoxin FldB -
BA058_RS07555 1585983..1586390 - 408 WP_000244765.1 protein YgfX Toxin
BA058_RS07560 1586371..1586637 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
BA058_RS07565 1586880..1587860 + 981 WP_000886080.1 tRNA-modifying protein YgfZ -
BA058_RS07570 1587937..1588596 - 660 WP_000250274.1 hemolysin III family protein -
BA058_RS07575 1588760..1589071 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
BA058_RS07580 1589116..1590549 + 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16043.95 Da        Isoelectric Point: 11.5202

>T64509 WP_000244765.1 NZ_CP016358:c1586390-1585983 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLIPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T64509 NZ_CP016358:c1586390-1585983 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATTCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT64509 WP_000354046.1 NZ_CP016358:c1586637-1586371 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT64509 NZ_CP016358:c1586637-1586371 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A454A7D7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References