Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2653632..2654283 Replicon chromosome
Accession NZ_CP016355
Organism Lacticaseibacillus paracasei subsp. paracasei strain TMW 1.1434

Toxin (Protein)


Gene name pemK Uniprot ID K6S8L5
Locus tag BBD24_RS13450 Protein ID WP_003567661.1
Coordinates 2653632..2654015 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A829TRS3
Locus tag BBD24_RS13455 Protein ID WP_003606045.1
Coordinates 2654035..2654283 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BBD24_RS13430 2650761..2651783 + 1023 WP_100908377.1 IS30 family transposase -
BBD24_RS13435 2651841..2652653 - 813 Protein_2428 glutamate synthase subunit alpha -
BBD24_RS13445 2652927..2653448 - 522 WP_100908647.1 QueT transporter family protein -
BBD24_RS13450 2653632..2654015 - 384 WP_003567661.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
BBD24_RS13455 2654035..2654283 - 249 WP_003606045.1 programmed cell death antitoxin Antitoxin
BBD24_RS13460 2654351..2655487 - 1137 WP_003606047.1 alanine racemase -
BBD24_RS13465 2655474..2655848 - 375 WP_003567669.1 holo-ACP synthase -
BBD24_RS13470 2656018..2657526 - 1509 WP_003567670.1 DEAD/DEAH box helicase -
BBD24_RS16300 2657652..2657795 - 144 WP_003591975.1 hypothetical protein -
BBD24_RS13475 2657826..2659214 - 1389 WP_003606050.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13773.03 Da        Isoelectric Point: 10.9764

>T64496 WP_003567661.1 NZ_CP016355:c2654015-2653632 [Lacticaseibacillus paracasei subsp. paracasei]
MDVSVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSTKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

>T64496 NZ_CP016355:c2654015-2653632 [Lacticaseibacillus paracasei subsp. paracasei]
ATGGATGTGTCCGTAAAACGCGGCGATGTTTTTTTCGCCGATCTGTCTCCGGTTGTCGGCTCCGAACAAGGCGGCAACCG
GCCAGTACTTATTATTCAAAACAACGTTGGCAACCATTACAGTCCCACAGTGATTGTGGCAGCCATTACCTCCAAGATTC
AAAAACCCAAGATGCCGACGCATGTCGGGTTACGGGCCAAGCAAGATGGCGTTGAACGCAACTCAGTCATTTTACTTGAG
CAAATTCGCACGATTGACAAGCAACGGCTACAAGCCCGCGTGACAGCTCTGTCAAGCACCAAAATGGCAGCAGTCGACCG
CGCGTTGGCGATTTCAGTGGGCTTGGTCAGTTTGCCCAAGCCCAAAACATATAATAAGAATTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8968.11 Da        Isoelectric Point: 4.4610

>AT64496 WP_003606045.1 NZ_CP016355:c2654283-2654035 [Lacticaseibacillus paracasei subsp. paracasei]
MKKQTSVAVTFDDAIKARLDAYCELNDIDAQTVITEAVATFLKSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp

>AT64496 NZ_CP016355:c2654283-2654035 [Lacticaseibacillus paracasei subsp. paracasei]
ATGAAAAAGCAAACGTCAGTAGCGGTAACGTTTGATGATGCCATTAAGGCGCGGTTGGATGCCTATTGTGAATTGAATGA
CATTGACGCTCAGACTGTGATTACGGAGGCAGTGGCGACATTTTTGAAGTCACAGGCTCCCGAAATCAACCAATTGATTT
CTGGCTATGTAGCAATGGGCCAGATCAATACAGAGATCAGTCATGCTTTCTCTGCCTGCGAGAGCGAGGCATACGCCCAT
ATTCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K6S8L5

Antitoxin

Source ID Structure
AlphaFold DB A0A829TRS3

References