Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4128305..4128924 Replicon chromosome
Accession NZ_CP016344
Organism Klebsiella variicola strain E57-7

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag BBB54_RS20470 Protein ID WP_002892050.1
Coordinates 4128706..4128924 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A1F2MBN7
Locus tag BBB54_RS20465 Protein ID WP_008805436.1
Coordinates 4128305..4128679 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BBB54_RS20450 4123460..4124653 + 1194 WP_012542667.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
BBB54_RS20455 4124676..4127822 + 3147 WP_008805437.1 multidrug efflux RND transporter permease subunit -
BBB54_RS20465 4128305..4128679 + 375 WP_008805436.1 Hha toxicity modulator TomB Antitoxin
BBB54_RS20470 4128706..4128924 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
BBB54_RS20475 4129083..4129649 + 567 WP_032438710.1 maltose O-acetyltransferase -
BBB54_RS27480 4129621..4129749 - 129 Protein_3914 hypothetical protein -
BBB54_RS20480 4129786..4130256 + 471 WP_008805434.1 YlaC family protein -
BBB54_RS20485 4130225..4131682 - 1458 WP_016160392.1 PLP-dependent aminotransferase family protein -
BBB54_RS20490 4131783..4132481 + 699 WP_012968815.1 GNAT family N-acetyltransferase -
BBB54_RS20495 4132478..4132618 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
BBB54_RS20500 4132618..4132881 - 264 WP_008805431.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T64489 WP_002892050.1 NZ_CP016344:4128706-4128924 [Klebsiella variicola]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T64489 NZ_CP016344:4128706-4128924 [Klebsiella variicola]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAACTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACCATGAATAAGCTGTACGATAAGATCCCGACCTCAGTCTGGAAGTTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14384.06 Da        Isoelectric Point: 4.8989

>AT64489 WP_008805436.1 NZ_CP016344:4128305-4128679 [Klebsiella variicola]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYTEDNKLVAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT64489 NZ_CP016344:4128305-4128679 [Klebsiella variicola]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACTTCGGCTGTCAATCTTCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCGCTTAATTATAAAATTAAGTACACTGAGGATAATAAACTGGTCGCTCAGGTTGACGAATATCTG
GACGATACTTTTACCTTGTTCAGTAACTACGGCATTAACTCAACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTCTCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2MBN7

References