Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2717100..2718688 Replicon chromosome
Accession NZ_CP016344
Organism Klebsiella variicola strain E57-7

Toxin (Protein)


Gene name hipA Uniprot ID A0A1F2MCM0
Locus tag BBB54_RS13480 Protein ID WP_008804919.1
Coordinates 2717100..2718422 (-) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A1F2MCN9
Locus tag BBB54_RS13485 Protein ID WP_008804920.1
Coordinates 2718422..2718688 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BBB54_RS13465 2712165..2714666 + 2502 WP_032754383.1 fimbrial biogenesis outer membrane usher protein -
BBB54_RS13470 2714654..2715658 + 1005 WP_032754382.1 type 1 fimbrial protein -
BBB54_RS13475 2715791..2717083 + 1293 WP_032754379.1 glycoside hydrolase family 10 protein -
BBB54_RS13480 2717100..2718422 - 1323 WP_008804919.1 type II toxin-antitoxin system HipA family toxin Toxin
BBB54_RS13485 2718422..2718688 - 267 WP_008804920.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
BBB54_RS13490 2718954..2719280 - 327 WP_008804921.1 hydroxyisourate hydrolase -
BBB54_RS13495 2719277..2719777 - 501 WP_032754377.1 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase -
BBB54_RS13500 2719755..2721134 - 1380 WP_012968246.1 purine permease -
BBB54_RS13505 2721479..2722633 + 1155 WP_032754375.1 FAD-dependent urate hydroxylase HpxO -
BBB54_RS13510 2722614..2723537 - 924 WP_032754373.1 LysR family hpxDE operon transcriptional regulator HpxR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(5-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(13-65)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 48911.26 Da        Isoelectric Point: 7.9109

>T64487 WP_008804919.1 NZ_CP016344:c2718422-2717100 [Klebsiella variicola]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGGHWCIPQGTTPTTHIIKLPIGEIKQPNATLDLRESVDNEFLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGIPSSMKYESDGGPGIAAIMAFLLGSSEALKDRADFMKFMVFQWLVGATDGHAKNFSLFLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPGEQMLAILQEFAGHVP
QAIESARRTLPADFSTHVWRAITENMLKLHARLQQGLQAL

Download         Length: 1323 bp

>T64487 NZ_CP016344:c2718422-2717100 [Klebsiella variicola]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGCACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCGCTGCCGCTGCAGTATGGCAATATCACCG
CCGACGCGGTGTATCACTATTTTGATAATCTGCTGCCCGACAGCCCGCAGGTCCGCGATCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTGGGACGGGATAGCGTAGGGGCGGTGACTCTGCTACCGCCGGG
GGAAGAGGCGCACCTTGAGGGGTTGCGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCCTACCAGT
CGGATATCCCTCTCGGGATGATTACCGGCCAGGATGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGGGCACTGGTGTATTCCGCAGGGCACCACGCCGACCACGCATATCATTAAACTGCCCATCGGCGAGAT
AAAGCAGCCTAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAACGAGTTTCTGTGCCTGGCGCTGGCCAGGGAACTGG
GCCTGGCGGTGCCGGAGGCAGAGATTATCACCACGCCGCGGATCCGCGCGCTGGCGGTGACCCGCTTTGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTACTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCCTTTGGCATCCCGTCGTCGATGAAATA
TGAATCTGACGGCGGGCCGGGTATTGCGGCAATCATGGCCTTTCTGCTGGGCTCCAGTGAAGCCCTGAAAGATCGCGCAG
ACTTTATGAAATTCATGGTTTTTCAGTGGCTGGTTGGGGCAACGGACGGGCATGCGAAGAACTTCTCGCTCTTTTTGCTG
CCCGGTGGCAGCTACCGTCTAACGCCGTTCTACGACATCATTTCGGCGTTTCCGGTGCTGGGAGGCACGGGACTGCATCT
GCGCGACCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGGAAAACAGAGATTAACGCTATCTATCCGCGTCATT
TTCTGGCAACCGCCAAAGCGGTGAACTTTCCTGGGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCACCCACGTCTGGCGGGCCATTACGGAAAATATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGTTATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9936.30 Da        Isoelectric Point: 8.5912

>AT64487 WP_008804920.1 NZ_CP016344:c2718688-2718422 [Klebsiella variicola]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKATETAPAP
DNNTDLDW

Download         Length: 267 bp

>AT64487 NZ_CP016344:c2718688-2718422 [Klebsiella variicola]
ATGAATTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTATCTGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACACTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTAGGGGTGGTGATGACGCTGAACGCCAAAGCCACAGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2MCM0

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2MCN9

References