Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 712300..713075 Replicon chromosome
Accession NZ_CP016344
Organism Klebsiella variicola strain E57-7

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A2N4Z6P0
Locus tag BBB54_RS03595 Protein ID WP_032740995.1
Coordinates 712590..713075 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag BBB54_RS03590 Protein ID WP_004150912.1
Coordinates 712300..712593 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BBB54_RS03570 707556..708158 - 603 WP_012540553.1 short chain dehydrogenase -
BBB54_RS03575 708256..709167 + 912 WP_012540554.1 LysR family transcriptional regulator -
BBB54_RS03580 709168..710316 - 1149 WP_008806488.1 PLP-dependent aspartate aminotransferase family protein -
BBB54_RS03585 710328..711704 - 1377 WP_016161835.1 cystathionine beta-synthase -
BBB54_RS03590 712300..712593 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
BBB54_RS03595 712590..713075 + 486 WP_032740995.1 GNAT family N-acetyltransferase Toxin
BBB54_RS03600 713779..714372 + 594 WP_004188553.1 hypothetical protein -
BBB54_RS03605 714469..714685 + 217 Protein_691 transposase -
BBB54_RS03620 715208..715921 - 714 WP_032736424.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 714469..714621 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17618.64 Da        Isoelectric Point: 8.5178

>T64476 WP_032740995.1 NZ_CP016344:712590-713075 [Klebsiella variicola]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVH
A

Download         Length: 486 bp

>T64476 NZ_CP016344:712590-713075 [Klebsiella variicola]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGACCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTCAT
GCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT64476 WP_004150912.1 NZ_CP016344:712300-712593 [Klebsiella variicola]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT64476 NZ_CP016344:712300-712593 [Klebsiella variicola]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACCGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2N4Z6P0

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References