Detailed information of TA system TA064464

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 3997629..3998189
• Replicon: chromosome
• Accession: NZ_CP016337
• Organism: Kosakonia sacchari strain BO-1

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: A0A1G4Y1N1
• Locus tag: BBB57_RS19070
• Protein ID: WP_017457648.1
• Coordinates: 3997629..3997940 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: BBB57_RS19075
• Protein ID: WP_065370129.1
• Coordinates: 3997944..3998189 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBB57_RS19050	3993162..3993737	+	576	WP_065370676.1	ABATE domain-containing protein	-
BBB57_RS19055	3994283..3995350	-	1068	WP_065370127.1	SDR family oxidoreductase	-
BBB57_RS19060	3995454..3996386	+	933	WP_065370128.1	LysR family transcriptional regulator	-
BBB57_RS19065	3996451..3997572	+	1122	WP_017457647.1	cupin domain-containing protein	-
BBB57_RS19070	3997629..3997940	-	312	WP_017457648.1	CcdB family protein	Toxin
BBB57_RS19075	3997944..3998189	-	246	WP_065370129.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
BBB57_RS19080	3998327..3998923	-	597	WP_197511314.1	nitroreductase family protein	-
BBB57_RS19085	3999806..4001131	+	1326	WP_065370130.1	sensor domain-containing diguanylate cyclase	-
BBB57_RS19090	4001152..4002294	-	1143	WP_065370131.1	PLP-dependent aspartate aminotransferase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-101)

Antitoxin

• Post-segregation antitoxin CcdA (11-81)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 11824.56 Da
• Isoelectric Point: 4.6436

>T64464 WP_017457648.1 NZ_CP016337:c3997940-3997629 [Kosakonia sacchari]
MQYSVYRNKSNSRDFPFLLDVQSDIIGSMNSRAVIPLCPLADYKDRRRVERLNPVLEIEGESYLLLTHDLAGVSLALLGE
EVCSMRAQRDVIRNAMDFIFDEI

Nucleotide

• Length: 312 bp

>T64464 NZ_CP016337:c3997940-3997629 [Kosakonia sacchari]
ATGCAATATTCGGTTTATCGGAACAAAAGTAACAGCCGGGATTTCCCGTTTCTGCTGGATGTACAGAGCGATATTATCGG
TAGCATGAACTCACGGGCGGTGATCCCGCTCTGTCCCCTGGCGGATTACAAAGATCGCCGCCGGGTTGAGCGGTTAAATC
CGGTACTGGAGATCGAAGGGGAATCGTATTTGTTACTGACCCACGATCTCGCAGGCGTCAGCCTGGCGCTTTTGGGTGAA
GAAGTTTGCAGTATGCGCGCCCAGCGCGACGTGATCCGCAATGCGATGGATTTTATTTTTGACGAGATTTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9305.38 Da
• Isoelectric Point: 7.2061

>AT64464 WP_065370129.1 NZ_CP016337:c3998189-3997944 [Kosakonia sacchari]
MPATATKQRNKQNVSVTVERDLLEQARKEGINLSQTLTNALQGELRNLEAERWKRENAAAIADLNEFIAERGHFSDEHRK
F

Nucleotide

• Length: 246 bp

>AT64464 NZ_CP016337:c3998189-3997944 [Kosakonia sacchari]
ATGCCTGCTACTGCAACTAAACAGCGAAACAAGCAAAATGTCAGCGTGACCGTCGAGCGCGACCTACTGGAACAAGCGCG
CAAAGAAGGCATTAACCTTTCGCAAACGCTGACGAACGCCCTGCAAGGCGAACTGCGTAATCTGGAAGCTGAGCGCTGGA
AGCGCGAAAATGCCGCCGCCATTGCCGATTTAAATGAATTTATTGCCGAACGCGGCCACTTCTCGGATGAACACAGGAAG
TTTTAA

Structures

Toxin

• AlphaFold DB: A0A1G4Y1N1