Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 2403195..2403768 Replicon chromosome
Accession NZ_CP016337
Organism Kosakonia sacchari strain BO-1

Toxin (Protein)


Gene name shpA Uniprot ID A0A1G4Y5H4
Locus tag BBB57_RS11465 Protein ID WP_017457810.1
Coordinates 2403487..2403768 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A1G4Y5V1
Locus tag BBB57_RS11460 Protein ID WP_017457811.1
Coordinates 2403195..2403500 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BBB57_RS11445 2399789..2401942 + 2154 WP_017457813.1 carbon starvation protein A -
BBB57_RS11450 2401996..2402199 + 204 WP_007373011.1 YbdD/YjiX family protein -
BBB57_RS11455 2402210..2403166 + 957 WP_065369361.1 GTPase -
BBB57_RS11460 2403195..2403500 - 306 WP_017457811.1 BrnA antitoxin family protein Antitoxin
BBB57_RS11465 2403487..2403768 - 282 WP_017457810.1 BrnT family toxin Toxin
BBB57_RS11470 2403904..2405274 - 1371 WP_065369362.1 NAD-dependent succinate-semialdehyde dehydrogenase -
BBB57_RS11475 2405847..2406362 + 516 WP_065369363.1 VOC family protein -
BBB57_RS11480 2406365..2407468 - 1104 WP_065369364.1 M20 peptidase aminoacylase family protein -
BBB57_RS11485 2407486..2407953 - 468 WP_020456432.1 YjiG family protein -
BBB57_RS11490 2407946..2408629 - 684 WP_017457805.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-86)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(40-93)


Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10928.36 Da        Isoelectric Point: 7.0746

>T64460 WP_017457810.1 NZ_CP016337:c2403768-2403487 [Kosakonia sacchari]
MEFEWDATKAISNLRKHGIRFEDAVLVFDDPQHLSIQDRFENGEYRWQTLGLVHGVVIILVAHTIRFESGHEVVRIISAR
KADRKERSRYEHG

Download         Length: 282 bp

>T64460 NZ_CP016337:c2403768-2403487 [Kosakonia sacchari]
ATGGAGTTCGAATGGGACGCAACAAAAGCGATTAGCAATCTGCGCAAGCACGGTATCCGCTTTGAAGATGCCGTATTGGT
TTTTGATGATCCGCAACATCTGTCGATACAAGACAGGTTTGAAAACGGCGAATATCGTTGGCAAACCCTCGGGCTGGTTC
ATGGCGTGGTCATTATTTTGGTCGCGCATACTATTCGTTTTGAAAGTGGGCACGAGGTCGTTCGTATTATCAGCGCGCGT
AAGGCTGACAGAAAGGAGAGGAGCCGTTATGAGCATGGTTAA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11673.44 Da        Isoelectric Point: 8.5038

>AT64460 WP_017457811.1 NZ_CP016337:c2403500-2403195 [Kosakonia sacchari]
MSMVKHKQGEMPVLNKQREAELQALADKPDDEIDYSDIPATEEALWADAVRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMMRDLQNKK

Download         Length: 306 bp

>AT64460 NZ_CP016337:c2403500-2403195 [Kosakonia sacchari]
ATGAGCATGGTTAAACATAAGCAGGGCGAGATGCCTGTGCTCAATAAGCAGCGTGAAGCTGAACTTCAAGCACTGGCAGA
TAAACCAGACGATGAGATTGATTACAGCGATATTCCTGCGACGGAAGAGGCGCTTTGGGCAGATGCGGTTCGCGGTAAAT
TTTTCCGCCCTTTAAAAACGCAGGCATCGGTGCGTATTGATGCGGATGTTATGGAATGGCTGAAGCGGCCAGGAAAAGGG
TATCAGACCCGGCTCAACGCAATTTTACGCGAAGCGATGATGCGCGATTTGCAAAACAAAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1G4Y5H4

Antitoxin

Source ID Structure
AlphaFold DB A0A1G4Y5V1

References