Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1901319..1901935 Replicon chromosome
Accession NZ_CP016337
Organism Kosakonia sacchari strain BO-1

Toxin (Protein)


Gene name Hha Uniprot ID A0A1G4ZCB1
Locus tag BBB57_RS09165 Protein ID WP_017459990.1
Coordinates 1901717..1901935 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A1G4ZCB5
Locus tag BBB57_RS09160 Protein ID WP_017459991.1
Coordinates 1901319..1901693 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BBB57_RS09150 1896425..1897618 + 1194 WP_065369141.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
BBB57_RS09155 1897641..1900790 + 3150 WP_065369142.1 multidrug efflux RND transporter permease subunit AcrB -
BBB57_RS09160 1901319..1901693 + 375 WP_017459991.1 Hha toxicity modulator TomB Antitoxin
BBB57_RS09165 1901717..1901935 + 219 WP_017459990.1 hemolysin expression modulator Hha Toxin
BBB57_RS09170 1902012..1902578 + 567 WP_065369143.1 maltose O-acetyltransferase -
BBB57_RS09175 1902679..1903158 + 480 WP_065369144.1 YlaC family protein -
BBB57_RS09180 1903118..1904572 - 1455 WP_065369145.1 PLP-dependent aminotransferase family protein -
BBB57_RS09185 1904672..1905373 + 702 WP_065369146.1 GNAT family N-acetyltransferase -
BBB57_RS09190 1905370..1905510 - 141 WP_025263710.1 type B 50S ribosomal protein L36 -
BBB57_RS09195 1905514..1905774 - 261 WP_017459984.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8651.08 Da        Isoelectric Point: 7.9893

>T64459 WP_017459990.1 NZ_CP016337:1901717-1901935 [Kosakonia sacchari]
MSEKPLTKIDYLMRLRRCQTIDTLERVIEKNKYELPDEELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Download         Length: 219 bp

>T64459 NZ_CP016337:1901717-1901935 [Kosakonia sacchari]
ATGTCCGAAAAACCATTAACAAAAATTGATTATTTGATGCGTTTAAGACGCTGTCAGACAATTGATACTCTTGAACGTGT
AATTGAAAAAAATAAATACGAACTTCCCGACGAGGAACTCGCGGTGTTTTATTCAGCAGCGGATCATCGTCTGGCAGAAC
TGACCATGAATAAGCTTTATGACAAAATCCCCTCCTCCGTGTGGAAGTTTGTACGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14550.41 Da        Isoelectric Point: 5.8346

>AT64459 WP_017459991.1 NZ_CP016337:1901319-1901693 [Kosakonia sacchari]
MDEYSPKRHDIAQLKFLCETLYHDCLVNLEESHHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIELIDEYL
DDTFMLFSSYGINTHDLQKWRKTGNKLFRSFVNVSKANPVSHSC

Download         Length: 375 bp

>AT64459 NZ_CP016337:1901319-1901693 [Kosakonia sacchari]
ATGGACGAATACTCGCCGAAAAGGCATGATATCGCGCAGCTCAAATTTCTCTGCGAAACGTTGTACCATGATTGCCTCGT
TAACCTTGAAGAGAGCCATCATGGGTGGGTTAACGATCCTACATCAGCAATCAATTTGCAGTTAAACGAGCTGATAGAAC
ACATAGCCACTTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTGATTGAACTGATAGACGAATACCTC
GACGATACGTTTATGTTGTTCAGTAGCTATGGCATAAATACACACGACTTACAGAAATGGCGTAAAACAGGTAACAAATT
GTTCCGCAGTTTCGTCAATGTAAGCAAGGCTAATCCTGTTAGCCACTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1G4ZCB1

Antitoxin

Source ID Structure
AlphaFold DB A0A1G4ZCB5

References