Detailed information of TA system TA064445

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): dhiT-phd/-
• Location: 446954..447358
• Replicon: chromosome
• Accession: NZ_CP016325
• Organism: Vibrio cholerae 2740-80

Toxin (Protein)

• Gene name: dhiT
• Uniprot ID: A0A086SLD6
• Locus tag: BBB50_RS16240
• Protein ID: WP_001114075.1
• Coordinates: 447089..447358 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: BBB50_RS20355
• Protein ID: WP_099607150.1
• Coordinates: 446954..447058 (+)
• Length: 35 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBB50_RS16200	442423..442710	+	288	WP_000426470.1	hypothetical protein	-
BBB50_RS16205	442851..443222	+	372	WP_001164080.1	nucleotide pyrophosphohydrolase	-
BBB50_RS20545	443289..443714	+	426	WP_001882332.1	DUF1778 domain-containing protein	-
BBB50_RS16215	443711..444244	+	534	WP_000118351.1	GNAT family N-acetyltransferase	-
BBB50_RS16220	444446..444703	+	258	WP_000861987.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
BBB50_RS16225	444691..444993	+	303	WP_000229317.1	type II toxin-antitoxin system RelE/ParE family toxin	-
BBB50_RS16230	445264..446148	+	885	WP_000842472.1	DUF3800 domain-containing protein	-
BBB50_RS16235	446298..446897	+	600	WP_001891245.1	glutathione S-transferase N-terminal domain-containing protein	-
BBB50_RS20355	446954..447058	+	105	WP_099607150.1	acetyltransferase	Antitoxin
BBB50_RS16240	447089..447358	+	270	WP_001114075.1	DUF4160 domain-containing protein	Toxin
BBB50_RS16245	447352..447873	+	522	WP_000921691.1	DUF2442 domain-containing protein	-
BBB50_RS20360	448172..448297	+	126	WP_001905700.1	DUF645 family protein	-
BBB50_RS16250	448548..448943	+	396	WP_001000867.1	hypothetical protein	-
BBB50_RS16255	449082..449360	-	279	WP_000578476.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	-
BBB50_RS16260	449357..449641	-	285	WP_000381183.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
BBB50_RS16265	449835..450350	+	516	WP_000343779.1	GNAT family N-acetyltransferase	-
BBB50_RS16270	450534..450947	+	414	WP_000049420.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	304243..451810	147567
	-	inside	Integron	-	-	309323..451434	142111

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF4160 (9-74)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10310.94 Da
• Isoelectric Point: 6.6303

>T64445 WP_001114075.1 NZ_CP016325:447089-447358 [Vibrio cholerae 2740-80]
MPEIDALLGLSFCLYFFDNKQHKLPHIHVKYGSYELIIAIETGECLEGYLPNKQRKRAENHILEHREQLMVMWNKAVNGE
NPGKLGDLC

Nucleotide

• Length: 270 bp

>T64445 NZ_CP016325:447089-447358 [Vibrio cholerae 2740-80]
ATGCCAGAGATTGATGCCCTATTAGGTCTTTCATTTTGCTTGTACTTCTTTGATAACAAGCAGCATAAATTACCTCATAT
TCACGTTAAGTACGGTAGTTACGAGCTAATTATTGCTATTGAAACAGGTGAGTGTTTAGAGGGTTACTTACCCAATAAGC
AACGAAAACGTGCTGAAAACCATATTCTTGAACATCGAGAGCAATTGATGGTGATGTGGAATAAAGCGGTGAATGGTGAA
AATCCAGGTAAGTTAGGTGATTTATGTTGA

Antitoxin

Protein

• Length: 35 a.a.
• Molecular weight: 3991.79 Da
• Isoelectric Point: 9.2853

>AT64445 WP_099607150.1 NZ_CP016325:446954-447058 [Vibrio cholerae 2740-80]
VVSVVVFEFSSMRCQPLRRALYAYRNCLLKDTLL

Nucleotide

• Length: 105 bp

>AT64445 NZ_CP016325:446954-447058 [Vibrio cholerae 2740-80]
GTGGTTTCGGTTGTTGTGTTTGAGTTTAGTAGTATGCGCTGCCAGCCCCTTAGGCGGGCGTTATATGCTTATAGGAATTG
TCTCCTAAAGGATACGCTGCTATAG

Structures

Toxin

• AlphaFold DB: A0A086SLD6