Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-Phd
Location 444446..444993 Replicon chromosome
Accession NZ_CP016325
Organism Vibrio cholerae 2740-80

Toxin (Protein)


Gene name relE Uniprot ID Q9KM92
Locus tag BBB50_RS16225 Protein ID WP_000229317.1
Coordinates 444691..444993 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID Q9KM93
Locus tag BBB50_RS16220 Protein ID WP_000861987.1
Coordinates 444446..444703 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BBB50_RS16180 439475..439930 + 456 WP_001245327.1 GNAT family N-acetyltransferase -
BBB50_RS20000 440252..440404 + 153 WP_001884520.1 DUF645 family protein -
BBB50_RS16185 440606..441103 - 498 WP_000982260.1 GNAT family N-acetyltransferase -
BBB50_RS16190 441100..441372 - 273 WP_000246253.1 DUF1778 domain-containing protein -
BBB50_RS16195 441603..442271 + 669 WP_000043871.1 hypothetical protein -
BBB50_RS16200 442423..442710 + 288 WP_000426470.1 hypothetical protein -
BBB50_RS16205 442851..443222 + 372 WP_001164080.1 nucleotide pyrophosphohydrolase -
BBB50_RS20545 443289..443714 + 426 WP_001882332.1 DUF1778 domain-containing protein -
BBB50_RS16215 443711..444244 + 534 WP_000118351.1 GNAT family N-acetyltransferase -
BBB50_RS16220 444446..444703 + 258 WP_000861987.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
BBB50_RS16225 444691..444993 + 303 WP_000229317.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BBB50_RS16230 445264..446148 + 885 WP_000842472.1 DUF3800 domain-containing protein -
BBB50_RS16235 446298..446897 + 600 WP_001891245.1 glutathione S-transferase N-terminal domain-containing protein -
BBB50_RS20355 446954..447058 + 105 WP_099607150.1 acetyltransferase -
BBB50_RS16240 447089..447358 + 270 WP_001114075.1 DUF4160 domain-containing protein -
BBB50_RS16245 447352..447873 + 522 WP_000921691.1 DUF2442 domain-containing protein -
BBB50_RS20360 448172..448297 + 126 WP_001905700.1 DUF645 family protein -
BBB50_RS16250 448548..448943 + 396 WP_001000867.1 hypothetical protein -
BBB50_RS16255 449082..449360 - 279 WP_000578476.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family -
BBB50_RS16260 449357..449641 - 285 WP_000381183.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 304243..451810 147567
- inside Integron - - 309323..451434 142111


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-69)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11726.68 Da        Isoelectric Point: 5.1972

>T64444 WP_000229317.1 NZ_CP016325:444691-444993 [Vibrio cholerae 2740-80]
MVEIIWTELALSDLNDIAEYIALENVVAAKQLVQTVFTKVERLADFPESGRVPPELEHLNYREVVVSPCRVFYKYDDAKV
RILFVMRAERDLRRLMLTKQ

Download         Length: 303 bp

>T64444 NZ_CP016325:444691-444993 [Vibrio cholerae 2740-80]
ATGGTTGAAATAATTTGGACGGAGCTGGCTCTATCCGACTTGAATGATATTGCTGAATACATCGCGCTTGAAAATGTCGT
GGCTGCTAAACAACTGGTGCAAACCGTTTTTACAAAAGTTGAACGTTTGGCCGATTTTCCAGAGTCTGGGCGCGTCCCTC
CAGAACTAGAACACCTCAATTATCGTGAAGTCGTTGTAAGTCCGTGTCGTGTTTTCTACAAGTATGATGATGCAAAGGTT
CGTATTCTTTTTGTTATGCGTGCGGAGCGAGATTTGCGTCGGTTAATGCTTACGAAACAGTAG

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9647.15 Da        Isoelectric Point: 7.3178

>AT64444 WP_000861987.1 NZ_CP016325:444446-444703 [Vibrio cholerae 2740-80]
MKVELVTSLKRQATKILADLHDTKEPVLITEHGKPSAYLIDVDDYEFMQNRLAILEGIARGERALADGKVVSHQDAKDRM
SKWLK

Download         Length: 258 bp

>AT64444 NZ_CP016325:444446-444703 [Vibrio cholerae 2740-80]
ATGAAAGTAGAACTTGTGACGTCACTAAAACGTCAGGCCACTAAGATCCTTGCCGATCTTCATGATACGAAAGAGCCAGT
ATTGATAACTGAGCACGGAAAACCGTCAGCTTACCTTATTGATGTAGACGACTATGAATTTATGCAAAATCGTTTAGCGA
TCCTGGAAGGTATTGCTCGCGGAGAGCGAGCTTTAGCGGATGGTAAAGTGGTCAGCCATCAAGATGCTAAGGACAGAATG
TCAAAATGGTTGAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q9KM92

Antitoxin

Source ID Structure
AlphaFold DB A0A1T4SLM9

References