Detailed information of TA system TA064443

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /DUF1778(antitoxin)
• Location: 443289..444244
• Replicon: chromosome
• Accession: NZ_CP016325
• Organism: Vibrio cholerae 2740-80

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KM94
• Locus tag: BBB50_RS16215
• Protein ID: WP_000118351.1
• Coordinates: 443711..444244 (+)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: BBB50_RS20545
• Protein ID: WP_001882332.1
• Coordinates: 443289..443714 (+)
• Length: 142 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBB50_RS16170	438819..439070	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
BBB50_RS16175	439060..439347	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
BBB50_RS16180	439475..439930	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-
BBB50_RS20000	440252..440404	+	153	WP_001884520.1	DUF645 family protein	-
BBB50_RS16185	440606..441103	-	498	WP_000982260.1	GNAT family N-acetyltransferase	-
BBB50_RS16190	441100..441372	-	273	WP_000246253.1	DUF1778 domain-containing protein	-
BBB50_RS16195	441603..442271	+	669	WP_000043871.1	hypothetical protein	-
BBB50_RS16200	442423..442710	+	288	WP_000426470.1	hypothetical protein	-
BBB50_RS16205	442851..443222	+	372	WP_001164080.1	nucleotide pyrophosphohydrolase	-
BBB50_RS20545	443289..443714	+	426	WP_001882332.1	DUF1778 domain-containing protein	Antitoxin
BBB50_RS16215	443711..444244	+	534	WP_000118351.1	GNAT family N-acetyltransferase	Toxin
BBB50_RS16220	444446..444703	+	258	WP_000861987.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
BBB50_RS16225	444691..444993	+	303	WP_000229317.1	type II toxin-antitoxin system RelE/ParE family toxin	-
BBB50_RS16230	445264..446148	+	885	WP_000842472.1	DUF3800 domain-containing protein	-
BBB50_RS16235	446298..446897	+	600	WP_001891245.1	glutathione S-transferase N-terminal domain-containing protein	-
BBB50_RS20355	446954..447058	+	105	WP_099607150.1	acetyltransferase	-
BBB50_RS16240	447089..447358	+	270	WP_001114075.1	DUF4160 domain-containing protein	-
BBB50_RS16245	447352..447873	+	522	WP_000921691.1	DUF2442 domain-containing protein	-
BBB50_RS20360	448172..448297	+	126	WP_001905700.1	DUF645 family protein	-
BBB50_RS16250	448548..448943	+	396	WP_001000867.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	304243..451810	147567
	-	inside	Integron	-	-	309323..451434	142111

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (97-153)

Antitoxin

• Vibrio phage ICP1, Orf50 (57-133)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 20171.46 Da
• Isoelectric Point: 9.1432

>T64443 WP_000118351.1 NZ_CP016325:443711-444244 [Vibrio cholerae 2740-80]
VSWGKEFVELSKSKHDRDSFDCGEQELNTFIKTQAAKHMQAGISRTMVLPSAHPLLNQKFAICAFYSVAPSSISRETLPA
QLAKKLPRYPIPVFLLAQLAVHKEFHGSGLGKVSLIRALKYLWEVNHHMRAYAIVVDCLTDSAQAFYTKFGFEVLCEHNE
RIRMFLPMKTVEQLFNQ

Nucleotide

• Length: 534 bp

>T64443 NZ_CP016325:443711-444244 [Vibrio cholerae 2740-80]
GTGAGTTGGGGCAAAGAGTTTGTAGAACTTAGTAAATCAAAGCATGACCGCGACTCATTTGACTGTGGTGAGCAAGAGCT
AAACACATTTATCAAAACTCAAGCTGCAAAGCACATGCAAGCAGGTATTAGCCGAACAATGGTTTTACCTAGTGCTCACC
CACTGTTAAATCAAAAATTCGCTATTTGTGCCTTTTATAGTGTTGCACCGAGCTCAATCAGCCGAGAAACATTACCGGCA
CAATTAGCAAAGAAACTACCGAGGTATCCAATCCCTGTTTTTCTGTTGGCTCAGTTAGCTGTGCACAAAGAATTTCACGG
TTCTGGACTTGGAAAGGTCAGTCTAATCCGTGCGCTGAAGTACTTGTGGGAAGTGAATCATCACATGAGAGCATACGCAA
TAGTTGTGGATTGTCTGACAGATTCCGCTCAAGCGTTCTATACGAAGTTCGGCTTTGAAGTATTGTGCGAACATAATGAG
CGTATTCGTATGTTCTTACCGATGAAAACGGTAGAACAACTTTTTAATCAATAG

Antitoxin

Protein

• Length: 142 a.a.
• Molecular weight: 15714.41 Da
• Isoelectric Point: 7.6659

>AT64443 WP_001882332.1 NZ_CP016325:443289-443714 [Vibrio cholerae 2740-80]
MLCLSLVVMRCQPLRRALYSPSPRKLSVRIECALSLAYNRSTDGMYPYAGGVMATARLDIRLDEEIKAKAEKASALLGLK
SLTEYVVRLMDEDATHVIEEHESIMVKDSVFDEFMAACNKVKAPNQALLEAVKFTEKSGIK

Nucleotide

• Length: 426 bp

>AT64443 NZ_CP016325:443289-443714 [Vibrio cholerae 2740-80]
TTGTTGTGTTTGAGTTTAGTGGTCATGCGTTGTCAGCCCCTTAGACGGGCGTTATATTCTCCCTCGCCCCGTAAACTTTC
CGTACGAATTGAATGTGCTTTAAGTTTGGCCTATAATCGCTCAACTGACGGAATGTATCCGTACGCAGGAGGGGTTATGG
CAACGGCACGTTTAGATATCCGCCTTGATGAAGAGATCAAGGCAAAAGCTGAAAAGGCATCGGCTTTACTTGGTTTAAAA
AGTCTCACTGAATATGTGGTGAGACTTATGGACGAAGATGCGACTCATGTTATTGAGGAGCATGAAAGTATTATGGTTAA
AGACAGTGTTTTTGACGAGTTTATGGCTGCGTGTAATAAAGTAAAAGCACCGAATCAAGCTTTGCTTGAAGCTGTTAAAT
TTACAGAGAAGAGCGGTATCAAGTGA

Structures

Toxin

• AlphaFold DB: Q9KM94