Detailed information of TA system TA064438

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 418113..418641
• Replicon: chromosome
• Accession: NZ_CP016325
• Organism: Vibrio cholerae 2740-80

Toxin (Protein)

• Gene name: relE
• Uniprot ID: H9L4R3
• Locus tag: BBB50_RS16010
• Protein ID: WP_000221352.1
• Coordinates: 418113..418403 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: H9L4T4
• Locus tag: BBB50_RS16015
• Protein ID: WP_000213183.1
• Coordinates: 418393..418641 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBB50_RS20515	413670..413795	+	126	WP_001944767.1	DUF645 family protein	-
BBB50_RS20520	413811..413849	+	39	WP_106019119.1	hypothetical protein	-
BBB50_RS15985	414062..415066	+	1005	WP_000964925.1	LD-carboxypeptidase	-
BBB50_RS15990	415219..415665	+	447	WP_000128977.1	hypothetical protein	-
BBB50_RS15995	415785..416714	+	930	WP_000335802.1	hypothetical protein	-
BBB50_RS20325	416711..416821	+	111	WP_071917832.1	DUF3265 domain-containing protein	-
BBB50_RS16000	416849..417325	+	477	WP_001047180.1	GNAT family N-acetyltransferase	-
BBB50_RS16005	417462..417977	+	516	WP_001201532.1	lipocalin family protein	-
BBB50_RS16010	418113..418403	-	291	WP_000221352.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BBB50_RS16015	418393..418641	-	249	WP_000213183.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
BBB50_RS16030	419212..419865	+	654	WP_000036957.1	site-specific DNA-methyltransferase	-
BBB50_RS16035	420137..420562	+	426	WP_000415748.1	hypothetical protein	-
BBB50_RS16040	420770..421165	+	396	WP_000649527.1	DUF3465 domain-containing protein	-
BBB50_RS16045	421316..421846	+	531	WP_000415563.1	hypothetical protein	-
BBB50_RS16050	421984..422484	+	501	WP_000789723.1	hypothetical protein	-
BBB50_RS16055	422640..423038	+	399	WP_001882293.1	hypothetical protein	-
BBB50_RS16060	423138..423602	-	465	WP_000140244.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	304243..451810	147567
	-	inside	Integron	-	-	309323..451434	142111

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11247.23 Da
• Isoelectric Point: 10.4678

>T64438 WP_000221352.1 NZ_CP016325:c418403-418113 [Vibrio cholerae 2740-80]
MTYKLEFKKSALKEWKKLAVPLQQQFKKKLIERLENPHVPSAKLSGAENIYKIKLRQSGYRLVYQVENDIIVVTVLAVGK
RERSEVYTKALQRLDD

Nucleotide

• Length: 291 bp

>T64438 NZ_CP016325:c418403-418113 [Vibrio cholerae 2740-80]
ATGACCTATAAGCTTGAGTTCAAAAAGAGTGCTTTGAAGGAATGGAAAAAGTTAGCCGTTCCACTCCAACAGCAGTTCAA
GAAGAAACTAATAGAACGCCTTGAAAATCCACATGTTCCATCAGCCAAACTGTCTGGCGCTGAGAACATTTATAAAATAA
AACTTCGGCAGTCTGGTTATCGCTTAGTCTATCAAGTTGAAAATGACATCATCGTAGTTACTGTTTTAGCTGTCGGTAAA
CGAGAACGCAGCGAAGTTTACACTAAAGCGCTGCAACGGCTAGACGACTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8937.28 Da
• Isoelectric Point: 4.1079

>AT64438 WP_000213183.1 NZ_CP016325:c418641-418393 [Vibrio cholerae 2740-80]
MTTRILADVAASITEFKANPMKVATSAFGAPVAVLNRNEPAFYCVPASTYEIMMDKLEDLELLAIAKERLSEDSVSVNID
DL

Nucleotide

• Length: 249 bp

>AT64438 NZ_CP016325:c418641-418393 [Vibrio cholerae 2740-80]
ATGACCACTAGAATCCTAGCTGATGTTGCAGCAAGCATTACCGAATTTAAAGCAAACCCAATGAAAGTTGCAACTAGTGC
CTTCGGTGCTCCTGTTGCTGTACTCAACCGAAATGAGCCGGCTTTCTACTGCGTCCCTGCAAGCACCTACGAGATCATGA
TGGATAAGCTTGAAGATCTTGAGCTACTGGCTATTGCCAAAGAACGCTTAAGTGAAGACAGCGTATCGGTAAATATCGAT
GACCTATAA

Structures

Toxin

• AlphaFold DB: H9L4R3

Antitoxin

• AlphaFold DB: A0A366A3W8