Detailed information of TA system TA064436

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-Phd
• Location: 379861..380439
• Replicon: chromosome
• Accession: NZ_CP016325
• Organism: Vibrio cholerae 2740-80

Toxin (Protein)

• Gene name: parE
• Uniprot ID: O68848
• Locus tag: BBB50_RS15740
• Protein ID: WP_001180243.1
• Coordinates: 379861..380178 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: Q7DCR7
• Locus tag: BBB50_RS15745
• Protein ID: WP_000557292.1
• Coordinates: 380197..380439 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBB50_RS15720	376169..376618	+	450	WP_001894449.1	hypothetical protein	-
BBB50_RS15735	378217..379224	+	1008	WP_000093800.1	DUF4365 domain-containing protein	-
BBB50_RS20265	379221..379331	+	111	Protein_366	DUF3265 domain-containing protein	-
BBB50_RS20490	379572..379667	+	96	WP_001907607.1	DUF645 family protein	-
BBB50_RS15740	379861..380178	-	318	WP_001180243.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BBB50_RS15745	380197..380439	-	243	WP_000557292.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
BBB50_RS15750	380681..381190	+	510	WP_000405987.1	GNAT family N-acetyltransferase	-
BBB50_RS15755	381247..381900	+	654	WP_000226874.1	hypothetical protein	-
BBB50_RS15760	382210..382428	+	219	WP_001917086.1	DUF3709 domain-containing protein	-
BBB50_RS19775	382831..383064	+	234	WP_032482776.1	DUF3709 domain-containing protein	-
BBB50_RS15765	383489..383669	+	181	Protein_374	DUF645 family protein	-
BBB50_RS15770	383936..384223	+	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	-
BBB50_RS15775	384234..384551	+	318	WP_001232701.1	HigA family addiction module antidote protein	-
BBB50_RS20495	384548..384658	+	111	WP_134814058.1	DUF3265 domain-containing protein	-
BBB50_RS19790	384835..384960	+	126	WP_001944767.1	DUF645 family protein	-
BBB50_RS20275	384976..385014	+	39	WP_106019119.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	304243..451810	147567
	-	inside	Integron	-	-	309323..451434	142111

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-93)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-71)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12158.97 Da
• Isoelectric Point: 9.7495

>T64436 WP_001180243.1 NZ_CP016325:c380178-379861 [Vibrio cholerae 2740-80]
MQNKQYKLSQLAQEHLLKIKHYTIENFAEAQWQKYKSTLLSGFQTLADNPGLGKSCEDIYQNGFYFPVGKHMAYYTKEAN
FILIVAVLGQSQLPQKHLKQSRFVS

Nucleotide

• Length: 318 bp

>T64436 NZ_CP016325:c380178-379861 [Vibrio cholerae 2740-80]
ATGCAAAATAAACAATATAAGCTCAGCCAATTAGCCCAAGAGCACTTACTCAAGATTAAACACTACACCATTGAAAATTT
CGCTGAAGCGCAGTGGCAAAAATATAAGTCGACCTTGCTTTCAGGTTTCCAAACTCTTGCGGATAACCCAGGACTAGGAA
AAAGCTGTGAAGATATTTACCAAAATGGTTTTTACTTTCCAGTGGGGAAACACATGGCTTATTACACCAAAGAAGCAAAC
TTCATTCTCATTGTTGCGGTATTAGGGCAATCACAACTGCCCCAAAAGCATCTCAAACAGTCACGCTTTGTTTCTTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8961.25 Da
• Isoelectric Point: 5.6630

>AT64436 WP_000557292.1 NZ_CP016325:c380439-380197 [Vibrio cholerae 2740-80]
MHTLTANDAKRNFGELLLSAQREPVIISKNSKNTVVVMSIKDFEELEAMKLDYLKHCFESAQKDLDSGKTVDGATFLNTL

Nucleotide

• Length: 243 bp

>AT64436 NZ_CP016325:c380439-380197 [Vibrio cholerae 2740-80]
ATGCACACACTAACAGCAAATGATGCTAAACGAAATTTTGGTGAACTTCTTCTAAGCGCACAACGCGAACCTGTCATCAT
CAGCAAAAACAGTAAGAATACTGTCGTTGTCATGTCCATTAAGGACTTTGAAGAACTTGAAGCAATGAAACTCGATTATC
TCAAACACTGCTTTGAGTCTGCTCAGAAAGACTTAGATAGTGGCAAAACCGTTGATGGTGCCACTTTTCTAAACACCCTT
TGA

Structures

Toxin

• AlphaFold DB: A0A0K9UJR8

Antitoxin

• AlphaFold DB: Q7DCR7