Detailed information of TA system TA064435

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 367103..367601
• Replicon: chromosome
• Accession: NZ_CP016325
• Organism: Vibrio cholerae 2740-80

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK7
• Locus tag: BBB50_RS15655
• Protein ID: WP_000589156.1
• Coordinates: 367103..367369 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: Q9KMK6
• Locus tag: BBB50_RS15660
• Protein ID: WP_000643598.1
• Coordinates: 367356..367601 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBB50_RS20245	362751..362911	+	161	Protein_340	DUF3709 domain-containing protein	-
BBB50_RS19700	362932..363153	+	222	WP_012705854.1	DUF3709 domain-containing protein	-
BBB50_RS15630	363473..363793	+	321	WP_001909340.1	DUF1778 domain-containing protein	-
BBB50_RS15635	363790..364323	+	534	WP_000118351.1	GNAT family N-acetyltransferase	-
BBB50_RS15640	364561..364839	+	279	WP_000280324.1	DUF3709 domain-containing protein	-
BBB50_RS20480	365214..365357	+	144	Protein_345	DUF645 family protein	-
BBB50_RS20485	365411..365449	+	39	WP_082798268.1	hypothetical protein	-
BBB50_RS15645	365661..366128	+	468	WP_001289288.1	OsmC family protein	-
BBB50_RS15650	366256..366918	+	663	WP_000960654.1	hypothetical protein	-
BBB50_RS15655	367103..367369	+	267	WP_000589156.1	BrnT family toxin	Toxin
BBB50_RS15660	367356..367601	+	246	WP_000643598.1	hypothetical protein	Antitoxin
BBB50_RS15665	367697..368491	+	795	WP_001911581.1	hypothetical protein	-
BBB50_RS15670	368649..369653	+	1005	WP_000964920.1	LD-carboxypeptidase	-
BBB50_RS15675	369941..370123	+	183	WP_000923182.1	DUF645 family protein	-
BBB50_RS15680	370320..370745	+	426	WP_000403005.1	GNAT family N-acetyltransferase	-
BBB50_RS15685	370885..371475	+	591	WP_000021480.1	hypothetical protein	-
BBB50_RS15690	371629..371988	+	360	WP_001071529.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	304243..451810	147567
	-	inside	Integron	-	-	309323..451434	142111

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-81)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10092.34 Da
• Isoelectric Point: 7.3171

>T64435 WP_000589156.1 NZ_CP016325:367103-367369 [Vibrio cholerae 2740-80]
MIKFEFDENKSRSNLEKHGIDFHTAQGLWNDSDLIEIPANTSDEPRYLVVGLLNGKHWSGVITYRGTNIRIISVRRSRKA
EVSLYESA

Nucleotide

• Length: 267 bp

>T64435 NZ_CP016325:367103-367369 [Vibrio cholerae 2740-80]
ATGATTAAATTTGAATTTGATGAAAACAAAAGCAGAAGTAATCTCGAAAAGCACGGTATTGATTTTCATACAGCTCAAGG
ATTGTGGAATGATTCAGATCTTATCGAGATCCCTGCTAACACGAGTGATGAGCCCAGATATTTGGTTGTCGGCTTACTAA
ACGGTAAGCATTGGTCAGGAGTAATTACCTACCGAGGTACGAATATCAGGATCATCTCAGTTCGGCGTTCACGGAAAGCG
GAGGTAAGCCTTTATGAAAGCGCATGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9478.77 Da
• Isoelectric Point: 6.4832

>AT64435 WP_000643598.1 NZ_CP016325:367356-367601 [Vibrio cholerae 2740-80]
MKAHEFDAKFESDDDDIVMDLDLSQAKRPMHKQKRVNVDFPAWMLESLDREASRIGVTRQSIIKIWLAERLESVSHHSSV
R

Nucleotide

• Length: 246 bp

>AT64435 NZ_CP016325:367356-367601 [Vibrio cholerae 2740-80]
ATGAAAGCGCATGAATTTGATGCCAAGTTTGAAAGTGACGACGATGATATCGTAATGGACTTGGATCTATCGCAAGCTAA
AAGACCGATGCATAAGCAAAAACGAGTCAATGTAGATTTTCCAGCCTGGATGCTTGAGTCCTTGGACAGAGAAGCGAGTC
GTATTGGTGTAACACGTCAATCAATCATTAAAATTTGGCTGGCAGAAAGGTTAGAGAGCGTGTCTCATCATTCAAGTGTG
AGATAA

Structures

Toxin

• AlphaFold DB: A0A271VMP7

Antitoxin

• AlphaFold DB: A0A271VME2