Detailed information of TA system TA064432

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-RHH
• Location: 336427..336965
• Replicon: chromosome
• Accession: NZ_CP016325
• Organism: Vibrio cholerae 2740-80

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A0K9UM73
• Locus tag: BBB50_RS15455
• Protein ID: WP_000802138.1
• Coordinates: 336427..336726 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A271VK51
• Locus tag: BBB50_RS15460
• Protein ID: WP_001107720.1
• Coordinates: 336723..336965 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBB50_RS15425	331770..331922	+	153	WP_001884520.1	DUF645 family protein	-
BBB50_RS15430	332124..332612	+	489	WP_016710990.1	hypothetical protein	-
BBB50_RS15435	332749..333225	+	477	WP_001894984.1	GNAT family N-acetyltransferase	-
BBB50_RS15440	333372..333908	+	537	WP_001083622.1	hypothetical protein	-
BBB50_RS20205	334005..334166	+	162	Protein_294	DUF3709 domain-containing protein	-
BBB50_RS19580	334160..334420	+	261	Protein_295	DUF3709 domain-containing protein	-
BBB50_RS15445	334697..335713	+	1017	WP_001894976.1	hypothetical protein	-
BBB50_RS15450	335858..336310	+	453	WP_000863110.1	GNAT family N-acetyltransferase	-
BBB50_RS15455	336427..336726	-	300	WP_000802138.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BBB50_RS15460	336723..336965	-	243	WP_001107720.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
BBB50_RS15465	337232..337945	+	714	WP_000123660.1	Fic family protein	-
BBB50_RS15470	338133..338648	+	516	WP_000284463.1	hypothetical protein	-
BBB50_RS15475	338767..339615	+	849	WP_000472050.1	hypothetical protein	-
BBB50_RS20210	339600..339728	+	129	WP_088126095.1	DUF3265 domain-containing protein	-
BBB50_RS15480	339765..340541	+	777	WP_001894915.1	hypothetical protein	-
BBB50_RS15485	340702..341322	+	621	WP_001173624.1	hypothetical protein	-
BBB50_RS19600	341849..341965	+	117	WP_080314581.1	DUF3265 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	304243..451810	147567
	-	inside	Integron	-	-	309323..451434	142111

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-92)

Antitoxin

• Antitoxin ParD (1-79)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11617.34 Da
• Isoelectric Point: 9.8184

>T64432 WP_000802138.1 NZ_CP016325:c336726-336427 [Vibrio cholerae 2740-80]
MKPFNLTVAAKADLRDIALFTQRRWGKEQRNVYLKQFDDSFWLLAENPDIGKSCDEIREGYRKYPQGSHVIFYQQIGSQQ
IRVIRILHKSMDVNPIFGA

Nucleotide

• Length: 300 bp

>T64432 NZ_CP016325:c336726-336427 [Vibrio cholerae 2740-80]
ATGAAACCATTTAATCTTACTGTCGCCGCTAAAGCCGATTTACGGGATATTGCTTTATTCACTCAACGACGCTGGGGAAA
AGAGCAGCGAAATGTTTATTTAAAGCAATTCGATGATTCCTTTTGGCTTTTAGCGGAAAATCCCGACATCGGTAAATCAT
GCGATGAAATTCGAGAGGGATACAGAAAATATCCCCAAGGGAGCCACGTCATCTTTTATCAGCAAATCGGCAGCCAACAA
ATCAGGGTGATCCGAATTCTTCATAAGAGCATGGATGTGAACCCAATATTTGGCGCATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8950.82 Da
• Isoelectric Point: 4.1677

>AT64432 WP_001107720.1 NZ_CP016325:c336965-336723 [Vibrio cholerae 2740-80]
MAKNTSITLGEHFDGFITSQIQSGRYGSASEVIRSALRLLENQETKLQSLRQLLVEGEQSGDADYDLDSFINELDSENIR

Nucleotide

• Length: 243 bp

>AT64432 NZ_CP016325:c336965-336723 [Vibrio cholerae 2740-80]
ATGGCTAAAAACACAAGTATCACTCTTGGTGAACACTTCGATGGCTTTATTACAAGCCAAATACAAAGTGGACGTTACGG
CTCAGCAAGTGAAGTCATTCGCTCTGCGCTACGTCTACTCGAAAACCAAGAAACCAAACTACAGTCACTCCGTCAATTAC
TTGTTGAAGGTGAGCAAAGTGGTGATGCTGATTATGACCTTGATAGCTTCATCAATGAACTCGATAGTGAAAACATTCGA
TGA

Structures

Toxin

• AlphaFold DB: A0A0K9UM73

Antitoxin

• AlphaFold DB: A0A271VK51