Detailed information of TA system TA064431

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-RelB
• Location: 326408..326948
• Replicon: chromosome
• Accession: NZ_CP016325
• Organism: Vibrio cholerae 2740-80

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A151JGK8
• Locus tag: BBB50_RS15390
• Protein ID: WP_000277238.1
• Coordinates: 326408..326677 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q9KML3
• Locus tag: BBB50_RS15395
• Protein ID: WP_001258569.1
• Coordinates: 326670..326948 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBB50_RS19550	321436..321579	+	144	WP_021448843.1	DUF3265 domain-containing protein	-
BBB50_RS15355	321614..322384	+	771	WP_000399050.1	hypothetical protein	-
BBB50_RS15360	322523..322906	+	384	WP_000473945.1	hypothetical protein	-
BBB50_RS19555	322935..323012	+	78	WP_080314602.1	DUF3265 domain-containing protein	-
BBB50_RS15365	323039..323512	+	474	WP_001894959.1	GrpB family protein	-
BBB50_RS15370	323653..324090	+	438	WP_001894912.1	hypothetical protein	-
BBB50_RS15380	324676..325191	+	516	WP_001894921.1	lipocalin family protein	-
BBB50_RS20465	325566..325676	+	111	WP_080314579.1	DUF645 family protein	-
BBB50_RS15385	326030..326211	+	182	Protein_281	DUF645 family protein	-
BBB50_RS15390	326408..326677	-	270	WP_000277238.1	type II toxin-antitoxin system YafQ family toxin	Toxin
BBB50_RS15395	326670..326948	-	279	WP_001258569.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
BBB50_RS19565	327303..327482	+	180	Protein_284	DUF645 family protein	-
BBB50_RS15400	327732..328445	+	714	WP_000123659.1	Fic family protein	-
BBB50_RS15405	328749..328919	+	171	WP_000483442.1	DUF645 family protein	-
BBB50_RS15410	329131..329754	+	624	WP_000247072.1	DUF1349 domain-containing protein	-
BBB50_RS15415	329911..330345	+	435	WP_000453680.1	GNAT family N-acetyltransferase	-
BBB50_RS15420	330538..331449	+	912	WP_000174843.1	hypothetical protein	-
BBB50_RS15425	331770..331922	+	153	WP_001884520.1	DUF645 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	304243..451810	147567
	-	inside	Integron	-	-	309323..451434	142111

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (2-89)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-85)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10406.11 Da
• Isoelectric Point: 9.5084

>T64431 WP_000277238.1 NZ_CP016325:c326677-326408 [Vibrio cholerae 2740-80]
MYKLEYSTQFKKDFKKITKMPISDIIEVGNVISKLQRGEKLEPKNVDHPLTGNWVGFRDCHIKPDLVLIYRVFNDQLQLA
RIGSHSDLF

Nucleotide

• Length: 270 bp

>T64431 NZ_CP016325:c326677-326408 [Vibrio cholerae 2740-80]
ATGTATAAACTCGAATACTCCACACAGTTTAAGAAAGATTTCAAAAAGATAACTAAGATGCCGATTTCAGACATCATTGA
AGTCGGCAATGTTATTTCTAAGCTGCAACGCGGCGAAAAGCTTGAACCCAAGAATGTTGACCATCCGTTAACTGGAAATT
GGGTTGGATTTCGAGACTGCCACATTAAACCCGACCTAGTGTTAATCTATCGAGTTTTTAACGATCAACTACAACTAGCG
CGCATTGGTTCACATAGCGATTTATTCTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10088.58 Da
• Isoelectric Point: 6.0728

>AT64431 WP_001258569.1 NZ_CP016325:c326948-326670 [Vibrio cholerae 2740-80]
MRTEMLSTRIDHDTKIAFTNVCDEMGLSTSQAIKLFAKAVINHGGIPFELRVPQPNEVTASAIQELVEGKGHKAESVEAM
LNELTEGKVKHV

Nucleotide

• Length: 279 bp

>AT64431 NZ_CP016325:c326948-326670 [Vibrio cholerae 2740-80]
ATGAGAACAGAAATGCTGAGCACGCGCATTGACCACGACACGAAAATTGCGTTCACAAACGTCTGCGATGAGATGGGTCT
AAGTACATCACAGGCCATTAAGTTATTTGCAAAAGCGGTTATTAATCATGGTGGAATCCCTTTTGAGCTGCGAGTTCCAC
AGCCTAATGAGGTTACTGCATCTGCAATTCAAGAGCTCGTTGAAGGAAAAGGACATAAAGCTGAATCCGTTGAAGCTATG
CTGAATGAGCTTACTGAAGGCAAAGTTAAGCATGTATAA

Structures

Toxin

• AlphaFold DB: A0A151JGK8

Antitoxin

• AlphaFold DB: A0A151KTP9