Detailed information of TA system TA064428

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 367889..368448
• Replicon: chromosome
• Accession: NZ_CP016322
• Organism: Vibrio vulnificus strain FORC_037

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: FORC37_RS17410
• Protein ID: WP_047111817.1
• Coordinates: 368170..368448 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A3Q0L0M2
• Locus tag: FORC37_RS17405
• Protein ID: WP_011082348.1
• Coordinates: 367889..368173 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC37_RS17375	362985..364178	+	1194	WP_088428330.1	glycine C-acetyltransferase	-
FORC37_RS17380	364264..365289	+	1026	WP_197672410.1	L-threonine 3-dehydrogenase	-
FORC37_RS17385	365369..365869	-	501	WP_065090958.1	hypothetical protein	-
FORC37_RS17390	365947..366210	-	264	WP_011082346.1	hypothetical protein	-
FORC37_RS17395	366395..366928	-	534	WP_088428331.1	hypothetical protein	-
FORC37_RS17400	367063..367671	-	609	WP_011151714.1	hypothetical protein	-
FORC37_RS17405	367889..368173	+	285	WP_011082348.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
FORC37_RS17410	368170..368448	+	279	WP_047111817.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
FORC37_RS24300	368477..368543	-	67	Protein_288	DUF3265 domain-containing protein	-
FORC37_RS17420	368603..370387	-	1785	WP_065090960.1	NACHT domain-containing protein	-
FORC37_RS17425	370432..370620	-	189	WP_088428622.1	DUF3265 domain-containing protein	-
FORC37_RS17430	370651..371619	+	969	WP_088428332.1	IS30-like element ISVa6 family transposase	-
FORC37_RS17435	371645..372205	-	561	WP_065090961.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	370843..371619	776
	-	inside	Integron	-	-	367063..370556	3493

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-89)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-45)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10951.63 Da
• Isoelectric Point: 4.3430

>T64428 WP_047111817.1 NZ_CP016322:368170-368448 [Vibrio vulnificus]
MILWEEESLNDREKIFEFLYDFNPDAAEKTDNLIETKVENLLEQPLMGVQRDSIRGRLLIIPEISMVISYWVEGDIIRVM
RVLHQKQKFPTD

Nucleotide

• Length: 279 bp

>T64428 NZ_CP016322:368170-368448 [Vibrio vulnificus]
ATGATTTTATGGGAAGAAGAGTCACTTAATGATCGTGAAAAGATCTTCGAGTTTCTCTATGACTTCAACCCTGATGCGGC
AGAAAAAACTGACAACCTCATTGAAACAAAAGTAGAAAACTTGCTTGAACAACCTTTAATGGGTGTACAACGAGATAGCA
TCCGCGGTCGATTACTCATTATTCCCGAGATTTCGATGGTTATCTCTTACTGGGTCGAGGGCGATATCATCCGAGTTATG
CGTGTCCTCCACCAGAAACAAAAATTCCCTACGGATTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 11083.43 Da
• Isoelectric Point: 9.2167

>AT64428 WP_011082348.1 NZ_CP016322:367889-368173 [Vibrio vulnificus]
MDTRIQFRVDEETKRLAQQMAESQGRTLSDACRELTEQLAEQQRKTLSHDAWLTEQVNLAFEKFDSGKSVFIEHQTAKSR
MEERKARIRNRGKQ

Nucleotide

• Length: 285 bp

>AT64428 NZ_CP016322:367889-368173 [Vibrio vulnificus]
ATGGACACTAGAATTCAATTTCGTGTTGATGAAGAAACAAAACGCCTAGCTCAACAAATGGCAGAGAGTCAAGGTCGCAC
TCTTAGTGACGCATGCCGTGAACTTACTGAGCAACTCGCTGAACAACAAAGAAAAACATTGTCTCACGATGCATGGCTAA
CTGAACAAGTAAATCTAGCATTTGAGAAGTTTGACTCAGGAAAATCCGTTTTCATTGAGCACCAAACTGCTAAATCTCGA
ATGGAAGAGCGCAAAGCCAGAATCCGTAATCGGGGTAAGCAATGA

Structures

Antitoxin

• AlphaFold DB: A0A3Q0L0M2