Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 244689..245331 Replicon chromosome
Accession NZ_CP016316
Organism Bacillus cereus strain M3

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag BA202_RS01425 Protein ID WP_000635963.1
Coordinates 244981..245331 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag BA202_RS01420 Protein ID WP_000004570.1
Coordinates 244689..244976 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BA202_RS01395 240015..240977 + 963 WP_000961156.1 UV DNA damage repair endonuclease UvsE -
BA202_RS01400 240970..241542 - 573 WP_016716299.1 rhomboid family intramembrane serine protease -
BA202_RS01405 241635..241994 + 360 WP_000635037.1 holo-ACP synthase -
BA202_RS01410 242151..243101 + 951 WP_029141035.1 outer membrane lipoprotein carrier protein LolA -
BA202_RS01415 243220..244389 + 1170 WP_000390608.1 alanine racemase -
BA202_RS01420 244689..244976 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
BA202_RS01425 244981..245331 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BA202_RS01430 245399..247567 + 2169 WP_061183209.1 RNA-binding transcriptional accessory protein -
BA202_RS01435 247625..247741 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
BA202_RS01440 247937..248395 + 459 WP_000344243.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T64397 WP_000635963.1 NZ_CP016316:244981-245331 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T64397 NZ_CP016316:244981-245331 [Bacillus cereus]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT64397 WP_000004570.1 NZ_CP016316:244689-244976 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT64397 NZ_CP016316:244689-244976 [Bacillus cereus]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References