Detailed information of TA system TA064396

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2386437..2387415
• Replicon: chromosome
• Accession: NZ_CP016315
• Organism: Bacillus cereus strain D12_2

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: W8Y388
• Locus tag: BA203_RS12350
• Protein ID: WP_000624977.1
• Coordinates: 2386437..2387174 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: W8YZL5
• Locus tag: BA203_RS12355
• Protein ID: WP_000237818.1
• Coordinates: 2387287..2387415 (+)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BA203_RS12325	2382079..2382861	+	783	WP_000381792.1	class I SAM-dependent methyltransferase	-
BA203_RS12330	2383019..2384704	-	1686	WP_033695152.1	alpha-keto acid decarboxylase family protein	-
BA203_RS12335	2384812..2385294	+	483	WP_000191886.1	MarR family winged helix-turn-helix transcriptional regulator	-
BA203_RS12340	2385461..2386198	+	738	WP_000594152.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
BA203_RS12350	2386437..2387174	+	738	WP_000624977.1	stage II sporulation protein SA	Toxin
BA203_RS12355	2387287..2387415	+	129	WP_000237818.1	hypothetical protein	Antitoxin
BA203_RS12360	2387488..2387664	+	177	WP_000852629.1	stage II sporulation protein SB	-
BA203_RS12365	2387682..2388071	-	390	WP_000713869.1	YxeA family protein	-
BA203_RS12370	2388283..2389752	+	1470	WP_048656315.1	beta-Ala-His dipeptidase	-
BA203_RS12375	2389998..2390807	+	810	WP_000678369.1	C39 family peptidase	-
BA203_RS12380	2390833..2391435	+	603	WP_048656314.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28307.03 Da
• Isoelectric Point: 8.2998

>T64396 WP_000624977.1 NZ_CP016315:2386437-2387174 [Bacillus cereus]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T64396 NZ_CP016315:2386437-2387174 [Bacillus cereus]
ATGATCTCTAACATTCGAATTGGCTTATTTGTTTTAGCGATTGTCTTTGTAGTCCTTGTTTTCTTTTATTGGAAAAATGA
GGAGTTGTACGAGGAGAAAAAGCAACGTATTAGAAAGACTTGGTATGGTTTGTTTATCATATCTGTCACCGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCAATGGTTATTTTTGTTGATATTGCGTTC
ATTTTAACGCCTAATATTTCAGAAATATGGGGTGCGAAATTTAGCGATATTGGCAAGACAGTTCAATCAATAAAACGATC
ATTAATTGCATCTAAAGCGAGAGGGGAAATATACACGACAATTATTCAAAATGTTAATCCAGCAGTATTCGGTACGATGG
AATGGCATACGGAAGAGGAATATACAAAGAGCTTAAATGCATTTTTAGATTCATATGGGGAAAAGATTGGCGCGAAAATT
GTTGTTTTTGAAGCCGCGAAAGAGTTAAATACAAACTTTCGTGGTATTCGCTCGCAATTTAGTATTATCGTTCCGTTAGA
ACATATCGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGGATTATACCAGCAAAAATAGTAAGTGACG
TTTTCATTGTTATTGATGGAAAGAAAAATAACCTGCAAGATCGGGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 5011.40 Da
• Isoelectric Point: 3.9150

>AT64396 WP_000237818.1 NZ_CP016315:2387287-2387415 [Bacillus cereus]
VVKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT64396 NZ_CP016315:2387287-2387415 [Bacillus cereus]
GTGGTAAAAGATTATGACAATGATTTTTTTAAAGAAGATAACGAAGATACAACAGATTTCTCTTTAATAAAAGGGGATAC
GTTGCAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATCATAA

Structures

Toxin

• AlphaFold DB: W8Y388

Antitoxin

• AlphaFold DB: W8YZL5