64393

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /GNAT-DUF1778
Location 1166650..1167416 Replicon chromosome
Accession NZ_CP016308
Organism Vibrio scophthalmi strain VS-12

Toxin (Protein)


Gene name - Uniprot ID A0A1B1NVV6
Locus tag VSVS12_RS20040 Protein ID WP_065431860.1
Coordinates 1166919..1167416 (+) Length 166 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A1B1NVP0
Locus tag VSVS12_RS20035 Protein ID WP_065431859.1
Coordinates 1166650..1166922 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
VSVS12_RS20015 1162769..1163620 - 852 WP_065431857.1 hypothetical protein -
VSVS12_RS20020 1163771..1164979 - 1209 WP_032073033.1 IS4 family transposase -
VSVS12_RS20025 1165102..1165854 - 753 WP_065431858.1 hypothetical protein -
VSVS12_RS20030 1166009..1166356 - 348 WP_060467711.1 DUF3024 domain-containing protein -
VSVS12_RS20035 1166650..1166922 + 273 WP_065431859.1 DUF1778 domain-containing protein Antitoxin
VSVS12_RS20040 1166919..1167416 + 498 WP_065431860.1 GNAT family N-acetyltransferase Toxin
VSVS12_RS20045 1167574..1168782 - 1209 WP_032073033.1 IS4 family transposase -
VSVS12_RS20050 1169331..1170317 - 987 WP_065431861.1 LacI family transcriptional regulator -
VSVS12_RS20055 1170552..1171991 + 1440 WP_065431862.1 sucrose-specific PTS transporter subunit IIBC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1166009..1185089 19080
- inside IScluster/Tn - - 1161285..1168782 7497
- inside Integron - - 1121237..1166922 45685


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(86-152)

Antitoxin

Vibrio phage ICP1, Orf50

(7-86)


Sequences

Toxin        


Download         Length: 166 a.a.        Molecular weight: 18583.28 Da        Isoelectric Point: 8.7753

>T64393 WP_065431860.1 NZ_CP016308:1166919-1167416 [Vibrio scophthalmi]
MMNTVLLEKAKHDRNRFNCGIEALNNYLKVMASQQAKKDNTRTFVLEDDNDSSHIIGFYTLTMTPIDLKALPDQLQKKHQ
SSTSGGLIARLAVDERYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKYFYERYGFQAFKEAENKLFITISDV
RANLG

Download         Length: 498 bp

>T64393 NZ_CP016308:1166919-1167416 [Vibrio scophthalmi]
ATGATGAATACGGTACTTCTAGAAAAAGCAAAACATGATAGAAACCGATTCAACTGTGGCATTGAAGCTCTTAATAACTA
TCTAAAAGTAATGGCGAGTCAACAAGCAAAGAAAGACAACACGCGAACTTTCGTTTTAGAAGATGATAACGATAGCTCGC
ACATCATTGGCTTTTACACGCTAACAATGACACCAATTGATTTAAAAGCATTACCTGACCAACTACAGAAAAAACACCAA
TCATCTACCTCTGGTGGTTTGATTGCCCGCTTAGCTGTAGATGAACGCTACAAAGGAAAAGGATTTGGCGAGTGGTTACT
TATCGATGCACTCCGAAAGCTACTAGCAGCCAGCGATAGTGTCGCATTCCCTGTAGTTATTGTTGACGCCAAAGACGGAG
CAAAATACTTCTATGAGCGCTATGGATTCCAAGCGTTTAAAGAGGCAGAAAACAAACTTTTTATTACCATTTCGGATGTA
AGAGCGAACTTAGGCTAG

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 9784.34 Da        Isoelectric Point: 9.6569

>AT64393 WP_065431859.1 NZ_CP016308:1166650-1166922 [Vibrio scophthalmi]
MATTLPRITARVDVDTQYLLTKAAAIAGMSSINSFVLSAAIEKAKQVIEREQALKLSQADAMLLMDALDRPATINSKLKA
ASERYESKTQ

Download         Length: 273 bp

>AT64393 NZ_CP016308:1166650-1166922 [Vibrio scophthalmi]
ATGGCGACTACTTTGCCTCGCATCACTGCTAGGGTTGATGTAGATACACAATACTTACTGACAAAAGCAGCTGCAATTGC
TGGTATGTCTAGTATCAACTCATTTGTTCTAAGCGCTGCTATCGAAAAAGCAAAACAAGTCATTGAACGTGAGCAAGCAC
TAAAACTTAGCCAAGCAGATGCCATGTTACTTATGGACGCTTTAGACCGTCCTGCGACAATAAACTCAAAATTGAAAGCA
GCTTCTGAACGTTACGAGAGCAAAACTCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B1NVV6

Antitoxin

Source ID Structure
AlphaFold DB A0A1B1NVP0

References