Detailed information of TA system TA064386

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 350968..351560
• Replicon: chromosome
• Accession: NZ_CP016303
• Organism: Candidatus Hamiltonella defensa (Bemisia tabaci) strain MEAM1

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A249DWF7
• Locus tag: BA171_RS01695
• Protein ID: WP_046493308.1
• Coordinates: 351264..351560 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: BA171_RS01690
• Protein ID: WP_016858108.1
• Coordinates: 350968..351267 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BA171_RS01650	346182..346517	-	336	WP_046493299.1	GPW/gp25 family protein	-
BA171_RS01655	346514..347092	-	579	WP_046493301.1	phage baseplate assembly protein V	-
BA171_RS01660	347082..347594	-	513	WP_029589149.1	DUF1834 family protein	-
BA171_RS01665	347621..348070	-	450	WP_046493304.1	phage virion morphogenesis protein	-
BA171_RS01670	348070..348504	-	435	WP_016858152.1	DUF1320 domain-containing protein	-
BA171_RS01675	348512..348856	-	345	WP_016858153.1	hypothetical protein	-
BA171_RS01680	348914..349834	-	921	WP_046493305.1	Mu-like prophage major head subunit gpT family protein	-
BA171_RS01685	349822..350673	-	852	WP_198303391.1	peptidase	-
BA171_RS08630	350690..350887	+	198	WP_198303388.1	hypothetical protein	-
BA171_RS01690	350968..351267	-	300	WP_016858108.1	putative addiction module antidote protein	Antitoxin
BA171_RS01695	351264..351560	-	297	WP_046493308.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BA171_RS08635	351602..352438	-	837	WP_198303392.1	DUF935 family protein	-
BA171_RS08640	352488..353240	-	753	WP_052734998.1	DUF935 family protein	-
BA171_RS01705	353243..354682	-	1440	WP_046493309.1	phage terminase large subunit	-
BA171_RS01710	354690..355195	-	506	Protein_329	hypothetical protein	-
BA171_RS01715	355179..355376	-	198	WP_016857916.1	hypothetical protein	-
BA171_RS01720	355380..355784	-	405	WP_016857915.1	hypothetical protein	-
BA171_RS01725	355784..356268	-	485	Protein_332	M15 family metallopeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	329474..369218	39744

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Uncharacterized protein HI1420 (6-93)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11268.02 Da
• Isoelectric Point: 10.1594

>T64386 WP_046493308.1 NZ_CP016303:c351560-351264 [Candidatus Hamiltonella defensa (Bemisia tabaci)]
MIELKQTETFRKWYTKLKDERATTAISLRLARLAYGHLGDAEPIGKGVSELRIHYGPGYRVYFQRRDAVIVILLCGGDKR
SQTRDIKTALRLAAEWSE

Nucleotide

• Length: 297 bp

>T64386 NZ_CP016303:c351560-351264 [Candidatus Hamiltonella defensa (Bemisia tabaci)]
ATGATCGAGTTGAAGCAGACTGAAACCTTTCGCAAATGGTATACCAAGCTCAAAGACGAGCGGGCGACCACAGCCATTTC
GTTGCGTCTGGCGCGGCTCGCCTACGGTCACCTCGGTGACGCCGAACCGATTGGCAAGGGTGTCAGTGAGCTTCGCATTC
ACTATGGCCCCGGCTACCGGGTATATTTCCAGCGACGTGACGCGGTGATCGTCATCCTGCTTTGCGGTGGCGACAAACGA
TCCCAGACCAGAGACATCAAAACTGCACTGCGCCTCGCTGCTGAATGGAGCGAATGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10637.31 Da
• Isoelectric Point: 7.2023

>AT64386 WP_016858108.1 NZ_CP016303:c351267-350968 [Candidatus Hamiltonella defensa (Bemisia tabaci)]
MTMTKKLTPFDPAEHLKSDQAIADFMAGAFETNDPGFIAHALGIIARAKGMTQIASQTGLSREQLYRSFSAEGNPTLRTT
LVLMKALGIELSAKPIAVD

Nucleotide

• Length: 300 bp

>AT64386 NZ_CP016303:c351267-350968 [Candidatus Hamiltonella defensa (Bemisia tabaci)]
ATGACCATGACTAAAAAACTGACCCCCTTCGACCCTGCTGAGCACCTGAAATCTGACCAAGCCATCGCTGACTTTATGGC
CGGTGCATTCGAGACAAACGATCCAGGATTTATTGCTCACGCTCTGGGCATCATCGCCCGTGCCAAAGGCATGACGCAAA
TCGCCAGCCAAACTGGCCTGTCACGTGAACAGCTTTATCGCTCGTTCAGCGCCGAGGGCAACCCGACTCTTCGCACGACA
CTGGTGCTGATGAAGGCACTGGGGATCGAATTGTCCGCGAAGCCGATCGCCGTTGACTGA

Structures

Toxin

• AlphaFold DB: A0A249DWF7