Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-Phd
Location 515360..516051 Replicon plasmid unnamed1
Accession NZ_CP016287
Organism Rhizobium leguminosarum strain Vaf10

Toxin (Protein)


Gene name vapC Uniprot ID A0A1B1CI32
Locus tag BA011_RS26875 Protein ID WP_065283023.1
Coordinates 515623..516051 (+) Length 143 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1B1CHY9
Locus tag BA011_RS26870 Protein ID WP_065283022.1
Coordinates 515360..515626 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BA011_RS26835 510383..510868 + 486 WP_065283018.1 DUF2384 domain-containing protein -
BA011_RS26840 510865..511371 + 507 WP_065283019.1 RES family NAD+ phosphorylase -
BA011_RS26845 511452..512177 - 726 WP_012755581.1 amino acid ABC transporter ATP-binding protein -
BA011_RS26850 512155..512814 - 660 WP_027664862.1 amino acid ABC transporter permease -
BA011_RS26855 512811..513482 - 672 WP_065283020.1 amino acid ABC transporter permease -
BA011_RS26860 513563..514348 - 786 WP_027664860.1 transporter substrate-binding domain-containing protein -
BA011_RS26865 514342..515103 - 762 WP_065283021.1 GntR family transcriptional regulator -
BA011_RS26870 515360..515626 + 267 WP_065283022.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
BA011_RS26875 515623..516051 + 429 WP_065283023.1 type II toxin-antitoxin system VapC family toxin Toxin
BA011_RS26880 516092..516862 - 771 WP_065283024.1 amino acid ABC transporter ATP-binding protein -
BA011_RS26885 516875..517534 - 660 WP_065283025.1 amino acid ABC transporter permease -
BA011_RS26890 517539..518201 - 663 WP_026264480.1 amino acid ABC transporter permease -
BA011_RS26895 518321..519160 - 840 WP_027664853.1 transporter substrate-binding domain-containing protein -
BA011_RS42305 519324..519539 - 216 Protein_499 PAS domain-containing protein -
BA011_RS26905 519526..519879 - 354 Protein_500 flagellar protein -
BA011_RS26910 519885..520532 - 648 WP_065283028.1 TetR/AcrR family transcriptional regulator -
BA011_RS42315 520915..521007 - 93 Protein_502 plasmid stabilization protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..1254530 1254530


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(9-66)


Sequences

Toxin        


Download         Length: 143 a.a.        Molecular weight: 15702.93 Da        Isoelectric Point: 4.6307

>T64371 WP_065283023.1 NZ_CP016287:515623-516051 [Rhizobium leguminosarum]
VRLLLDTNVLSEVTRPRPDAYVLQWLDGLDEDRSFISVVSIAETRRGVALMESGRKCDALAEWLAQDLPQRFEQRVIPVD
QPVATAWGDLMGLAKRSGRGLSSMDGLIAATAIAHGLTLATRNIKDFEGFGIELVDPWTERP

Download         Length: 429 bp

>T64371 NZ_CP016287:515623-516051 [Rhizobium leguminosarum]
GTGAGACTTCTGCTGGACACCAATGTGCTTTCCGAGGTGACAAGGCCGCGTCCCGATGCATATGTCCTACAATGGCTGGA
CGGTCTCGACGAGGATCGCTCATTCATCAGCGTCGTATCGATCGCGGAGACCCGGCGTGGTGTTGCATTGATGGAAAGCG
GTCGGAAGTGCGACGCGCTGGCTGAATGGCTTGCCCAGGACCTGCCGCAGCGCTTCGAGCAGCGTGTCATACCTGTGGAC
CAGCCGGTTGCGACAGCCTGGGGCGACTTGATGGGGCTCGCAAAGCGAAGCGGACGCGGCTTGTCGTCAATGGACGGCCT
GATTGCTGCCACCGCGATTGCTCACGGCCTCACCCTCGCCACTCGCAATATAAAGGATTTCGAGGGTTTTGGGATCGAGC
TGGTCGATCCTTGGACCGAGCGCCCGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9986.38 Da        Isoelectric Point: 10.4718

>AT64371 WP_065283022.1 NZ_CP016287:515360-515626 [Rhizobium leguminosarum]
MQPSRPEDKSWTVANAKAKLSEVIERAQATPQTIMRNGKPSVVVVSAEEWQRKTARKGTLAEFLMQSPLRGADLDLERQR
DEPRNLQL

Download         Length: 267 bp

>AT64371 NZ_CP016287:515360-515626 [Rhizobium leguminosarum]
ATGCAGCCTTCACGACCAGAGGATAAAAGTTGGACCGTCGCCAATGCGAAGGCCAAGCTGTCTGAGGTTATCGAACGCGC
GCAAGCAACGCCGCAAACGATCATGCGCAACGGTAAACCAAGCGTCGTCGTCGTTTCGGCCGAGGAATGGCAGCGGAAGA
CCGCCCGCAAGGGCACCCTTGCAGAGTTTCTGATGCAATCGCCGCTGCGTGGCGCCGATCTGGACCTCGAACGGCAGCGC
GACGAACCGCGTAATCTCCAGCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B1CI32

Antitoxin

Source ID Structure
AlphaFold DB A0A1B1CHY9

References