Detailed information of TA system TA064368

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 4621547..4622338
• Replicon: chromosome
• Accession: NZ_CP016286
• Organism: Rhizobium leguminosarum strain Vaf10

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: BA011_RS22485
• Protein ID: WP_151343509.1
• Coordinates: 4621547..4621966 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: A0A1B1CEP1
• Locus tag: BA011_RS22490
• Protein ID: WP_065282076.1
• Coordinates: 4622063..4622338 (-)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BA011_RS22470	4618479..4618859	-	381	WP_065282072.1	GFA family protein	-
BA011_RS22475	4618915..4620405	-	1491	WP_065282073.1	MFS transporter	-
BA011_RS22480	4620665..4621036	-	372	WP_065282074.1	hypothetical protein	-
BA011_RS22485	4621547..4621966	-	420	WP_151343509.1	GNAT family N-acetyltransferase	Toxin
BA011_RS22490	4622063..4622338	-	276	WP_065282076.1	DUF1778 domain-containing protein	Antitoxin
BA011_RS22500	4622978..4623937	-	960	WP_065282077.1	nitronate monooxygenase family protein	-
BA011_RS22505	4624224..4625423	+	1200	WP_065282078.1	regulator	-
BA011_RS22510	4625513..4626394	-	882	WP_065282079.1	polyphosphate kinase 2	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (14-115)

Antitoxin

• Vibrio phage ICP1, Orf50 (11-84)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15262.57 Da
• Isoelectric Point: 9.4643

>T64368 WP_151343509.1 NZ_CP016286:c4621966-4621547 [Rhizobium leguminosarum]
MALYNQEQGYTRTFVIADENFRVVGYHSICAGMIHRNDVSRSVKGSKSPTEIPIAVLARLAVDADHQGRGLGKVLLKNAL
LSVVSAAQVVAFRAVIIHALDDDAVTFYKKFGFQETKNMERRLILPTKDIAASLNETTG

Nucleotide

• Length: 420 bp

>T64368 NZ_CP016286:c4621966-4621547 [Rhizobium leguminosarum]
ATGGCCTTATATAATCAGGAGCAGGGATATACTCGTACATTTGTAATTGCGGACGAAAACTTCAGAGTGGTAGGTTACCA
TTCGATTTGCGCAGGTATGATACACCGAAACGATGTTTCCCGATCGGTAAAGGGGTCTAAATCTCCCACCGAAATTCCGA
TTGCAGTTTTAGCCCGACTTGCGGTTGATGCCGATCATCAAGGTAGGGGCCTTGGCAAAGTACTCCTTAAAAACGCGCTC
CTGAGTGTGGTTTCCGCCGCGCAGGTAGTCGCTTTTCGCGCCGTGATAATCCACGCTCTCGATGATGATGCTGTCACTTT
TTATAAAAAATTCGGCTTTCAGGAGACGAAGAACATGGAGCGGCGTCTCATTTTGCCGACAAAGGACATTGCAGCTTCCT
TGAACGAGACCACAGGCTAG

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10087.53 Da
• Isoelectric Point: 4.5994

>AT64368 WP_065282076.1 NZ_CP016286:c4622338-4622063 [Rhizobium leguminosarum]
MSALRKTKTVNLRIEPETHDLIARAAEVCGKSITAFMTEASVYTAQEELLDQRFIGVSAEVFDAVSDQLAAPGVARDNLV
KLFQTKVEWMD

Nucleotide

• Length: 276 bp

>AT64368 NZ_CP016286:c4622338-4622063 [Rhizobium leguminosarum]
ATGTCCGCTCTTAGGAAAACCAAAACCGTCAATCTTCGAATTGAACCGGAAACGCATGATCTAATCGCACGCGCTGCTGA
GGTTTGTGGAAAGAGCATAACTGCGTTCATGACCGAAGCCTCTGTATATACCGCGCAAGAAGAGTTGCTAGATCAACGAT
TCATAGGTGTATCCGCTGAAGTGTTTGACGCTGTAAGTGACCAGCTTGCCGCTCCAGGCGTAGCCAGAGACAACTTGGTC
AAGCTTTTTCAGACCAAAGTAGAATGGATGGACTGA

Structures

Antitoxin

• AlphaFold DB: A0A1B1CEP1