Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-couple_hipB
Location 177912..179401 Replicon chromosome
Accession NZ_CP016286
Organism Rhizobium leguminosarum strain Vaf10

Toxin (Protein)


Gene name HipA1 Uniprot ID A0A1B1C3X4
Locus tag BA011_RS00855 Protein ID WP_065279082.1
Coordinates 177912..179174 (-) Length 421 a.a.

Antitoxin (Protein)


Gene name HipB1 Uniprot ID A0A1B1C3T1
Locus tag BA011_RS00860 Protein ID WP_065279083.1
Coordinates 179171..179401 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BA011_RS00835 173340..174035 + 696 WP_151343365.1 hypothetical protein -
BA011_RS00840 174170..175708 - 1539 WP_065279080.1 GNAT family N-acetyltransferase -
BA011_RS00845 175683..176474 - 792 WP_065282355.1 helix-turn-helix domain-containing protein -
BA011_RS00850 176709..177488 - 780 WP_065279081.1 SDR family NAD(P)-dependent oxidoreductase -
BA011_RS00855 177912..179174 - 1263 WP_065279082.1 type II toxin-antitoxin system HipA family toxin Toxin
BA011_RS00860 179171..179401 - 231 WP_065279083.1 helix-turn-helix transcriptional regulator Antitoxin
BA011_RS00865 179790..180398 - 609 WP_065279084.1 TetR/AcrR family transcriptional regulator -
BA011_RS00870 180510..181196 + 687 WP_065279085.1 PspA/IM30 family protein -
BA011_RS00875 181246..181527 + 282 WP_003546080.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(6-104)

Antitoxin

Cro/C1-type helix-turn-helix domain

(12-69)


Sequences

Toxin        


Download         Length: 421 a.a.        Molecular weight: 45465.19 Da        Isoelectric Point: 5.1068

>T64364 WP_065279082.1 NZ_CP016286:c179174-177912 [Rhizobium leguminosarum]
MTSRLLNVWWDGRIVGQFTQDRDGDIGFVYDEDWLDDDKTLPLSASLPKRPERFTRRECRPYFAGLLPEESQRLAAARAL
GVSPANDFALLDHLGGDVAGALQLLAEGGAPAETGSIADRQPVPLDEAGIVAVLEALPVRPLLAGEEGLRLSLAGAQSKL
PVVLVDGRIGLPLPGQATTHILKPPIARFKATTENEAFVMRLAAAIGLDVAAVEPRYVQGRPFLLIERYDRVRGAGGLVH
RIHQEDFCQALGVPPETKYASEGGPTFKDCFDLIRRISARPAVDVLKLLDAAIFNLIAGNADAHGKNFSILYDSQGPRLA
KLYDLLSTMIYPELSPKLAMRLGKRATLAEMDAEGWKAFAAEAGVGTPLLRRRVNELCDKVLTLTGKVADELADVSPDRD
PIDAIAGVITDRAETARLTL

Download         Length: 1263 bp

>T64364 NZ_CP016286:c179174-177912 [Rhizobium leguminosarum]
ATGACATCAAGGCTTCTCAACGTCTGGTGGGACGGCCGCATTGTCGGTCAATTCACGCAGGACAGAGACGGCGATATCGG
CTTTGTCTATGATGAAGACTGGCTTGACGATGACAAAACGCTGCCGCTCTCGGCGTCTCTGCCGAAACGACCGGAGCGAT
TTACCCGCCGGGAATGCCGGCCGTATTTCGCCGGCCTGCTGCCCGAGGAAAGCCAGCGCCTGGCCGCAGCACGGGCCTTG
GGCGTGTCTCCCGCCAATGACTTTGCGCTTCTCGATCACCTGGGCGGCGATGTCGCCGGTGCGCTGCAACTGCTTGCCGA
AGGTGGGGCACCGGCGGAGACAGGGTCGATTGCAGATCGCCAGCCCGTGCCCTTGGATGAAGCAGGCATCGTTGCCGTGT
TGGAGGCACTGCCCGTGCGGCCTCTGCTGGCCGGTGAGGAGGGCCTGAGACTATCTCTCGCCGGCGCCCAGTCCAAACTG
CCGGTTGTTCTGGTGGATGGCCGGATCGGGCTTCCCCTGCCCGGTCAGGCCACAACCCATATTCTGAAGCCACCGATAGC
CCGCTTCAAGGCGACGACCGAGAACGAAGCTTTCGTGATGAGGCTTGCGGCCGCCATCGGGCTCGATGTCGCAGCCGTCG
AACCGAGATATGTGCAGGGACGCCCGTTCCTTCTGATCGAACGATATGACCGCGTGCGCGGCGCCGGCGGACTGGTTCAT
CGCATCCATCAGGAAGATTTCTGCCAGGCACTCGGCGTACCGCCGGAAACGAAATATGCGAGTGAAGGCGGGCCGACCTT
CAAGGATTGCTTCGATCTCATAAGACGGATTTCCGCAAGGCCGGCTGTCGACGTGCTGAAGCTGCTGGACGCGGCCATCT
TCAACCTGATCGCCGGAAATGCCGATGCCCACGGCAAGAACTTCTCCATCCTTTACGATAGCCAAGGCCCGCGGCTGGCA
AAACTCTATGACCTCCTCTCCACCATGATCTATCCGGAACTGTCGCCAAAGCTGGCGATGAGATTAGGCAAGCGCGCCAC
ACTGGCCGAAATGGATGCCGAGGGCTGGAAGGCCTTTGCGGCAGAAGCCGGCGTCGGCACTCCGCTGTTGCGCCGCCGCG
TGAACGAGCTTTGCGACAAGGTGCTGACATTGACCGGCAAGGTCGCCGATGAGCTCGCAGACGTATCGCCCGATCGAGAC
CCGATCGACGCGATCGCCGGCGTGATAACAGACCGGGCCGAGACAGCGAGGTTGACTTTATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8133.43 Da        Isoelectric Point: 9.3464

>AT64364 WP_065279083.1 NZ_CP016286:c179401-179171 [Rhizobium leguminosarum]
MAKHVQSPADIGALVRSTRKEQNLRQDELAGVAGVGLRFIVDLEAGKPTAQIGKVLQVLQTLGCSLDILAPGERRQ

Download         Length: 231 bp

>AT64364 NZ_CP016286:c179401-179171 [Rhizobium leguminosarum]
ATGGCGAAACACGTACAGAGTCCAGCCGACATCGGCGCCCTGGTTCGAAGCACCCGCAAGGAACAAAACCTGCGGCAGGA
CGAGCTTGCCGGCGTTGCCGGCGTCGGGCTTCGTTTTATCGTCGACCTCGAAGCCGGCAAGCCCACAGCGCAGATCGGCA
AGGTCCTGCAGGTTCTGCAAACGCTTGGCTGCTCCCTCGACATTCTGGCTCCCGGCGAGCGCAGGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B1C3X4

Antitoxin

Source ID Structure
AlphaFold DB A0A1B1C3T1

References