Detailed information of TA system TA064360

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-CC2985
• Location: 46518..47052
• Replicon: plasmid pCD1
• Accession: NZ_CP016274
• Organism: Yersinia pestis strain Cadman

Toxin (Protein)

• Gene name: parE
• Uniprot ID: Q663N8
• Locus tag: BAY22_RS21565
• Protein ID: WP_002213360.1
• Coordinates: 46518..46817 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: Q9ZGW3
• Locus tag: BAY22_RS21570
• Protein ID: WP_002224342.1
• Coordinates: 46810..47052 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BAY22_RS21535	42207..43229	+	1023	WP_000255944.1	IS21-like element IS100 family transposase	-
BAY22_RS21540	43229..44008	+	780	WP_001297096.1	IS21-like element IS100kyp family helper ATPase IstB	-
BAY22_RS24670	44075..44323	-	249	Protein_55	IS21 family transposase	-
BAY22_RS21550	44426..45070	+	645	Protein_56	IS3 family transposase	-
BAY22_RS21555	45133..45525	-	393	WP_002213403.1	type III secretion system chaperone SycE/YerA	-
BAY22_RS21560	45719..46378	+	660	WP_002229754.1	type III secretion system effector GTPase activator YopE	-
BAY22_RS21565	46518..46817	-	300	WP_002213360.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BAY22_RS21570	46810..47052	-	243	WP_002224342.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
BAY22_RS24100	47218..47510	-	293	Protein_61	IS6 family transposase	-
BAY22_RS25100	47596..47769	+	174	WP_002213357.1	hypothetical protein	-
BAY22_RS24825	47918..48154	-	237	WP_154020274.1	hypothetical protein	-
BAY22_RS21580	48314..49279	-	966	WP_002213354.1	ParB/RepB/Spo0J family plasmid partition protein	-
BAY22_RS21585	49276..50442	-	1167	WP_002220902.1	plasmid-partitioning protein SopA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	yscN / yscN / yscO / yscP / yscQ / yscR / yscS / yscT / yscU / virG/yscW / yscB / yscC / yscD / yscE / yscF / yscG / yopR/yscH/lcrP / lcrO/yscI / ylpB/yscJ / yscK / yscL / lcrQ/yscM / yopH / yopJ/yopP / yopO/ypkA / yadA / yadA / sycH / sycE/yerA / yopE / yopK/yopQ / yopT / sycT / yopM / yopD / yopB / sycD/lcrH / lcrV / lcrG / lcrR / yscV/lcrD / yscY / yscX / sycN / tyeA / lcrE/yopN	1..70304	70304

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-89)

Antitoxin

• Antitoxin ParD (5-72)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11632.19 Da
• Isoelectric Point: 5.5114

>T64360 WP_002213360.1 NZ_CP016274:c46817-46518 [Yersinia pestis]
VYKLSELADEDIYNIASYTIRHFGVTQAKLYHENLAKVFELLAKNPELGAECNWICSDIRRFQYKKHGIYYITLSNDILI
SRVLHQSIDIDVQDFPEHE

Nucleotide

• Length: 300 bp

>T64360 NZ_CP016274:c46817-46518 [Yersinia pestis]
GTGTATAAACTGTCTGAACTAGCTGATGAGGATATTTATAATATTGCCAGTTATACTATCCGGCATTTCGGTGTGACTCA
GGCTAAGTTATACCATGAGAACTTGGCAAAGGTATTCGAGCTATTAGCTAAAAATCCAGAGTTAGGGGCTGAGTGTAACT
GGATTTGCTCTGATATTCGCCGTTTTCAGTATAAAAAGCACGGTATATACTATATAACGCTTAGCAATGATATTTTGATT
TCTCGAGTGCTGCATCAATCCATAGATATAGATGTTCAGGATTTTCCAGAGCATGAGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8865.15 Da
• Isoelectric Point: 6.4781

>AT64360 WP_002224342.1 NZ_CP016274:c47052-46810 [Yersinia pestis]
MAHVTSVTLGEHLTGFVGEMIQSGRYGNISEVLRDALRLMEAREQRVQHVRDMVLAGTNVPVSHRLMDEIFSAAVKDTSV

Nucleotide

• Length: 243 bp

>AT64360 NZ_CP016274:c47052-46810 [Yersinia pestis]
ATGGCTCACGTAACCAGCGTAACACTTGGAGAGCATTTGACAGGTTTTGTGGGGGAAATGATTCAGTCTGGTCGTTATGG
CAATATATCAGAAGTGCTTCGTGATGCCTTAAGGCTAATGGAAGCACGTGAACAGCGTGTTCAACATGTACGCGATATGG
TGCTTGCAGGAACAAATGTACCTGTGAGCCATCGTTTAATGGATGAGATTTTTTCTGCTGCGGTGAAAGATACTAGTGTA
TAA

Structures

Toxin

• AlphaFold DB: Q663N8

Antitoxin

• AlphaFold DB: A0A0E1NQY6