Detailed information of TA system TA064332

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 3586453..3587336
• Replicon: chromosome
• Accession: NZ_CP016212
• Organism: Pseudomonas putida JB

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A5F0WHQ8
• Locus tag: Q5O_RS16285
• Protein ID: WP_070384038.1
• Coordinates: 3586453..3586890 (-)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A524BX56
• Locus tag: Q5O_RS16290
• Protein ID: WP_014591577.1
• Coordinates: 3586887..3587336 (-)
• Length: 150 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Q5O_RS16265	3581591..3582655	+	1065	WP_014591573.1	TerC family protein	-
Q5O_RS16270	3582751..3583980	-	1230	WP_070384037.1	acyl-CoA dehydrogenase	-
Q5O_RS16275	3584121..3585047	+	927	WP_014591574.1	LysR family transcriptional regulator	-
Q5O_RS16280	3585241..3586395	+	1155	WP_070384484.1	aminotransferase class V-fold PLP-dependent enzyme	-
Q5O_RS16285	3586453..3586890	-	438	WP_070384038.1	RES family NAD+ phosphorylase	Toxin
Q5O_RS16290	3586887..3587336	-	450	WP_014591577.1	DUF2384 domain-containing protein	Antitoxin
Q5O_RS16295	3587478..3588131	-	654	WP_014591578.1	oxygen-insensitive NAD(P)H-dependent nitroreductase NfsB	-
Q5O_RS16300	3588235..3589158	+	924	WP_014591579.1	LysR family transcriptional regulator	-
Q5O_RS16305	3589207..3590037	+	831	WP_070384039.1	AraC family transcriptional regulator	-
Q5O_RS16310	3590088..3590672	+	585	WP_014591580.1	LysE family transporter	-
Q5O_RS16315	3590712..3591911	-	1200	WP_014591581.1	sugar transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-138)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (97-146)

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15972.26 Da
• Isoelectric Point: 6.2214

>T64332 WP_070384038.1 NZ_CP016212:c3586890-3586453 [Pseudomonas putida JB]
VILWRISAYADLSGTGGLRVSGRWHQAGRPVVYAATSPPGAMLEVLVHLEIDPEDFPTTMRLLRIELPDTVSQAQLPALR
PGWSAQPELTRTLGNRFLDDGSALLLPVPSAIMPSTTNYLFNPRHPQAQSAKIQVEDFTPDSRLF

Nucleotide

• Length: 438 bp

>T64332 NZ_CP016212:c3586890-3586453 [Pseudomonas putida JB]
GTGATTTTGTGGCGAATCAGCGCCTATGCAGACTTGAGCGGTACGGGGGGCTTGCGTGTAAGTGGCCGCTGGCACCAGGC
GGGTCGGCCAGTGGTGTATGCCGCCACCAGCCCTCCCGGGGCGATGCTGGAAGTATTGGTGCACCTGGAGATCGACCCCG
AGGATTTCCCGACCACCATGCGCTTGCTTCGCATCGAACTGCCCGACACGGTTTCCCAGGCGCAGTTGCCCGCTTTGCGG
CCCGGCTGGTCTGCTCAGCCTGAGTTGACCCGTACGCTGGGCAATCGCTTTCTGGATGACGGTTCGGCATTGCTGCTGCC
GGTGCCAAGCGCGATCATGCCCAGCACCACCAATTACCTGTTCAACCCACGGCACCCACAGGCGCAGAGCGCGAAAATCC
AGGTTGAGGATTTCACGCCGGACAGCCGCCTGTTCTAG

Antitoxin

Protein

• Length: 150 a.a.
• Molecular weight: 16984.51 Da
• Isoelectric Point: 5.7258

>AT64332 WP_014591577.1 NZ_CP016212:c3587336-3586887 [Pseudomonas putida JB]
MLAEVLRDNGYHEYRARLQALLDIPELASDFEIHTRITDGFAATWLVKLTERGVLTPVERDQIIPLRTLKSRIERDQPLT
VDESDRLFRSAHITAMAEAVFGEVGKAKRWLSKPKERFSGLTPMQMLTTQQGTTQVEEMLLQIAEGYGL

Nucleotide

• Length: 450 bp

>AT64332 NZ_CP016212:c3587336-3586887 [Pseudomonas putida JB]
ATGCTTGCCGAAGTGCTACGCGACAACGGTTACCACGAGTACCGGGCGCGCTTGCAGGCGCTGCTGGACATTCCCGAGCT
TGCCAGCGACTTCGAAATCCACACCCGCATCACCGATGGTTTTGCCGCCACCTGGTTGGTAAAGCTGACTGAACGCGGTG
TGCTCACGCCGGTGGAGCGCGACCAGATCATTCCGCTGCGCACCCTCAAATCCCGTATCGAGCGTGACCAACCGCTGACC
GTGGATGAGAGCGACCGGCTGTTCCGCTCGGCCCACATCACCGCCATGGCCGAGGCTGTGTTCGGGGAAGTGGGCAAGGC
CAAGCGCTGGCTTTCCAAGCCTAAGGAACGCTTCTCGGGGCTGACGCCGATGCAGATGCTCACCACCCAGCAAGGCACCA
CGCAGGTCGAAGAAATGCTGCTGCAGATTGCCGAGGGCTATGGCCTGTGA

Structures

Toxin

• AlphaFold DB: A0A5F0WHQ8

Antitoxin

• AlphaFold DB: A0A524BX56