Detailed information of TA system TA064331

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2161445..2162101
• Replicon: chromosome
• Accession: NZ_CP016212
• Organism: Pseudomonas putida JB

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: Q5O_RS09630
• Protein ID: WP_024718231.1
• Coordinates: 2161919..2162101 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: Q5O_RS09625
• Protein ID: WP_070383674.1
• Coordinates: 2161445..2161870 (-)
• Length: 142 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Q5O_RS09580	2156496..2157323	+	828	WP_070383667.1	Rha family transcriptional regulator	-
Q5O_RS09585	2157323..2158097	+	775	Protein_1888	Pyocin large subunit-like protein	-
Q5O_RS09590	2158094..2158855	+	762	WP_070383668.1	Replication protein P	-
Q5O_RS09600	2159060..2159458	+	399	WP_070383670.1	recombination protein NinB	-
Q5O_RS09605	2159458..2159760	+	303	WP_070383671.1	DUF1364 family protein	-
Q5O_RS09610	2159757..2160116	+	360	WP_070383672.1	endodeoxyribonuclease RusA	-
Q5O_RS27870	2160113..2160259	+	147	WP_172830916.1	hypothetical protein	-
Q5O_RS09615	2160256..2160936	+	681	WP_053867845.1	hypothetical protein	-
Q5O_RS09625	2161445..2161870	-	426	WP_070383674.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
Q5O_RS09630	2161919..2162101	-	183	WP_024718231.1	type II toxin-antitoxin system HicA family toxin	Toxin
Q5O_RS09635	2162198..2162509	+	312	WP_053867848.1	hypothetical protein	-
Q5O_RS09640	2162509..2162778	+	270	WP_012053005.1	hypothetical protein	-
Q5O_RS09645	2162874..2163344	+	471	WP_053867849.1	DUF1311 domain-containing protein	-
Q5O_RS09650	2163405..2163596	+	192	WP_053867850.1	hypothetical protein	-
Q5O_RS09655	2163593..2163841	+	249	WP_053867851.1	hypothetical protein	-
Q5O_RS09660	2163873..2164076	-	204	WP_053867852.1	hypothetical protein	-
Q5O_RS09665	2164481..2165089	+	609	WP_053867853.1	hypothetical protein	-
Q5O_RS09670	2165121..2165597	+	477	WP_070383675.1	DUF2280 domain-containing protein	-
Q5O_RS09675	2165572..2166900	+	1329	WP_053867855.1	terminase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2137446..2208127	70681

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-66)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 7042.10 Da
• Isoelectric Point: 10.6600

>T64331 WP_024718231.1 NZ_CP016212:c2162101-2161919 [Pseudomonas putida JB]
MKYSEFRRWLEAQGVEFSKSANGSHFKIRYKDRQTIFPSHGSKEIGEGLRKEIIKQLGLK

Nucleotide

• Length: 183 bp

>T64331 NZ_CP016212:c2162101-2161919 [Pseudomonas putida JB]
ATGAAGTACAGCGAGTTCAGGAGATGGTTGGAGGCCCAAGGGGTCGAGTTCTCGAAGTCAGCCAACGGCAGCCACTTCAA
GATTCGCTACAAGGACCGCCAGACGATCTTCCCGAGCCACGGATCCAAGGAGATTGGTGAAGGACTCAGGAAGGAAATAA
TCAAGCAACTGGGCCTCAAATGA

Antitoxin

Protein

• Length: 142 a.a.
• Molecular weight: 15236.32 Da
• Isoelectric Point: 4.7662

>AT64331 WP_070383674.1 NZ_CP016212:c2161870-2161445 [Pseudomonas putida JB]
MYDYKIVAHEENDHFWSSCPDIPEAHSVGDSLEELLANAVDGLTLALSIYVDQKRAIPPATEAGDHIVRLSGVTVAKIAL
WNELVRSGKTRADLASMLGISPTAAGRLVDFEHTSKLESLEEALAKFGVRLQVIPTALQAA

Nucleotide

• Length: 426 bp

>AT64331 NZ_CP016212:c2161870-2161445 [Pseudomonas putida JB]
ATGTACGACTACAAAATCGTGGCTCACGAAGAAAACGATCACTTCTGGTCGTCTTGCCCAGACATCCCTGAGGCTCACAG
CGTGGGCGATAGCCTGGAAGAGCTTCTGGCAAATGCGGTGGACGGGCTCACCCTGGCGCTGTCGATCTACGTGGACCAGA
AGCGGGCAATTCCACCTGCCACCGAAGCCGGCGATCACATCGTTCGGCTTTCTGGCGTAACGGTCGCAAAAATCGCACTC
TGGAATGAGCTGGTCCGCTCCGGCAAGACCCGGGCTGACCTAGCCTCGATGCTCGGCATCTCCCCTACCGCAGCCGGGCG
CTTGGTCGACTTCGAGCACACCTCGAAGCTCGAGAGCCTGGAAGAAGCTTTGGCCAAGTTCGGAGTTCGACTCCAAGTGA
TACCTACCGCGCTGCAGGCTGCTTGA