Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4307002..4307578 Replicon chromosome
Accession NZ_CP016182
Organism Escherichia coli strain EC590

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag BAY41_RS22150 Protein ID WP_001297643.1
Coordinates 4307291..4307578 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag BAY41_RS22145 Protein ID WP_000063156.1
Coordinates 4307002..4307304 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BAY41_RS22130 4303598..4305748 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
BAY41_RS22135 4305798..4306001 + 204 WP_000467859.1 YbdD/YjiX family protein -
BAY41_RS22140 4306012..4306968 + 957 WP_001297640.1 GTPase -
BAY41_RS22145 4307002..4307304 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
BAY41_RS22150 4307291..4307578 - 288 WP_001297643.1 BrnT family toxin Toxin
BAY41_RS22155 4307777..4308474 - 698 Protein_4043 IS1 family transposase -
BAY41_RS22160 4308531..4308626 + 96 Protein_4044 dienelactone hydrolase family protein -
BAY41_RS22165 4308772..4310004 + 1233 WP_001380906.1 multidrug efflux MFS transporter MdtM -
BAY41_RS22170 4310045..4311325 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T64326 WP_001297643.1 NZ_CP016182:c4307578-4307291 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T64326 NZ_CP016182:c4307578-4307291 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT64326 WP_000063156.1 NZ_CP016182:c4307304-4307002 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT64326 NZ_CP016182:c4307304-4307002 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References