Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2621760..2622286 Replicon chromosome
Accession NZ_CP016182
Organism Escherichia coli strain EC590

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag BAY41_RS13370 Protein ID WP_000323025.1
Coordinates 2621999..2622286 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag BAY41_RS13365 Protein ID WP_000534858.1
Coordinates 2621760..2621999 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BAY41_RS13325 2617488..2617721 - 234 WP_001341330.1 IS66 family insertion sequence element accessory protein TnpB -
BAY41_RS13330 2617769..2617867 - 99 Protein_2433 IS66 family insertion sequence element accessory protein TnpB -
BAY41_RS24605 2618089..2618376 + 288 Protein_2434 hypothetical protein -
BAY41_RS13350 2619145..2620458 + 1314 WP_000589005.1 ISNCY family transposase -
BAY41_RS13355 2620895..2621227 - 333 WP_001301033.1 protein FlxA -
BAY41_RS13360 2621430..2621735 - 306 WP_001326990.1 hypothetical protein -
BAY41_RS13365 2621760..2621999 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
BAY41_RS13370 2621999..2622286 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
BAY41_RS13375 2622358..2622513 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
BAY41_RS13380 2622730..2622981 + 252 WP_000980994.1 hypothetical protein -
BAY41_RS13385 2623048..2623326 + 279 WP_012304870.1 hypothetical protein -
BAY41_RS13390 2623328..2624377 + 1050 WP_001265199.1 DUF968 domain-containing protein -
BAY41_RS13395 2624391..2625143 + 753 WP_001047135.1 antitermination protein -
BAY41_RS24390 2625421..2625510 - 90 WP_120795389.1 hypothetical protein -
BAY41_RS13400 2625565..2625777 - 213 WP_000087756.1 cold shock-like protein CspF -
BAY41_RS13405 2626078..2626293 + 216 WP_000066484.1 cold shock-like protein CspB -
BAY41_RS24735 2626657..2626827 - 171 WP_211180497.1 putative zinc-binding protein YnfU -
BAY41_RS13410 2627047..2627262 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2606190..2639261 33071


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T64320 WP_000323025.1 NZ_CP016182:2621999-2622286 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T64320 NZ_CP016182:2621999-2622286 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT64320 WP_000534858.1 NZ_CP016182:2621760-2621999 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT64320 NZ_CP016182:2621760-2621999 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References