Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Txe-Axe/Txe-RelB
Location 38257..38776 Replicon plasmid ISMMS_VRE11_p3
Accession NZ_CP016166
Organism Enterococcus faecium strain ISMMS_VRE_11

Toxin (Protein)


Gene name Txe Uniprot ID Q848U6
Locus tag AL025_RS16120 Protein ID WP_000588503.1
Coordinates 38519..38776 (+) Length 86 a.a.

Antitoxin (Protein)


Gene name Axe Uniprot ID Q83WJ8
Locus tag AL025_RS16115 Protein ID WP_000388479.1
Coordinates 38257..38526 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AL025_RS16095 33321..33884 - 564 WP_000017403.1 recombinase family protein -
AL025_RS16100 34527..35480 + 954 WP_000222571.1 IS30 family transposase -
AL025_RS16105 35458..36933 + 1476 WP_000443455.1 RNA-directed DNA polymerase -
AL025_RS16110 37208..37888 - 681 WP_001015311.1 IS6-like element IS1216 family transposase -
AL025_RS16785 37950..38126 + 177 WP_193402197.1 hypothetical protein -
AL025_RS16115 38257..38526 + 270 WP_000388479.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
AL025_RS16120 38519..38776 + 258 WP_000588503.1 Txe/YoeB family addiction module toxin Toxin
AL025_RS16125 39235..40239 + 1005 WP_000455809.1 hypothetical protein -
AL025_RS16130 40404..41018 - 615 WP_001261742.1 recombinase family protein -
AL025_RS16135 41468..42793 + 1326 WP_000969590.1 Y-family DNA polymerase -
AL025_RS16140 42786..43136 + 351 WP_000568378.1 hypothetical protein -
AL025_RS16145 43133..43312 + 180 WP_001021554.1 hypothetical protein -
AL025_RS16150 43448..43738 + 291 WP_000026576.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid erm(B) / ant(6)-Ia / aph(3')-III / VanHAX - 1..48256 48256


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-85)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-72)


Sequences

Toxin        


Download         Length: 86 a.a.        Molecular weight: 10206.53 Da        Isoelectric Point: 8.4369

>T64271 WP_000588503.1 NZ_CP016166:38519-38776 [Enterococcus faecium]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY

Download         Length: 258 bp

>T64271 NZ_CP016166:38519-38776 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10373.51 Da        Isoelectric Point: 4.4346

>AT64271 WP_000388479.1 NZ_CP016166:38257-38526 [Enterococcus faecium]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD

Download         Length: 270 bp

>AT64271 NZ_CP016166:38257-38526 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A828ZXQ8

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZWU7

References