Detailed information of TA system TA064266

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 4994484..4995158
• Replicon: chromosome
• Accession: NZ_CP016162
• Organism: Pseudomonas alcaliphila JAB1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: UYA_RS23345
• Protein ID: WP_075750565.1
• Coordinates: 4994976..4995158 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: UYA_RS23340
• Protein ID: WP_075750563.1
• Coordinates: 4994484..4994888 (-)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
UYA_RS23315	4990050..4990802	+	753	WP_021490505.1	ABC transporter substrate-binding protein	-
UYA_RS23320	4990857..4991552	+	696	WP_075750559.1	ABC transporter permease	-
UYA_RS23325	4991549..4992238	+	690	WP_045735745.1	ABC transporter permease	-
UYA_RS23330	4992291..4993505	+	1215	WP_075750561.1	methyltransferase	-
UYA_RS23340	4994484..4994888	-	405	WP_075750563.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
UYA_RS23345	4994976..4995158	-	183	WP_075750565.1	type II toxin-antitoxin system HicA family toxin	Toxin
UYA_RS23350	4995612..4996391	-	780	WP_075750567.1	sulfite exporter TauE/SafE family protein	-
UYA_RS23355	4996388..4997176	-	789	WP_075750569.1	3'(2'),5'-bisphosphate nucleotidase CysQ	-
UYA_RS23360	4997246..4998715	+	1470	WP_083665779.1	sulfate adenylyltransferase subunit CysD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6750.79 Da
• Isoelectric Point: 10.3586

>T64266 WP_075750565.1 NZ_CP016162:c4995158-4994976 [Pseudomonas alcaliphila JAB1]
MRSREVIELIEADGWYEVAVKGSHHQFKHPTKPGRVTVPHPKSEIAVGTLHNILKQAGLR

Nucleotide

• Length: 183 bp

>T64266 NZ_CP016162:c4995158-4994976 [Pseudomonas alcaliphila JAB1]
ATGCGAAGTAGGGAGGTGATCGAGCTAATTGAGGCGGATGGGTGGTACGAGGTGGCGGTGAAAGGCAGTCATCATCAGTT
CAAGCATCCAACCAAGCCAGGCCGGGTGACTGTTCCACACCCCAAGTCAGAGATAGCGGTCGGAACGCTGCACAACATAC
TGAAACAAGCTGGCCTCAGGTGA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14325.09 Da
• Isoelectric Point: 4.4520

>AT64266 WP_075750563.1 NZ_CP016162:c4994888-4994484 [Pseudomonas alcaliphila JAB1]
MKFPVVLHKDADSDYGVTVPDVPGCFSAGATVSEALDNAQEALALHFEGLVADGDPLPRAQEVDAHMGNPDYAGGVWAVV
DFDVTPYLGKAVRFNATLPENLLQRIDDVVKKDHRYASRSGFLAAAALRELSVA

Nucleotide

• Length: 405 bp

>AT64266 NZ_CP016162:c4994888-4994484 [Pseudomonas alcaliphila JAB1]
ATGAAATTTCCAGTCGTGCTGCACAAGGACGCTGATTCGGACTATGGGGTGACTGTGCCCGATGTACCGGGCTGTTTCTC
GGCAGGCGCCACTGTCTCGGAGGCTCTGGACAACGCACAGGAAGCCCTGGCCCTTCACTTTGAAGGATTGGTGGCTGATG
GTGATCCGCTCCCGCGTGCGCAGGAAGTAGACGCGCATATGGGGAATCCCGACTATGCCGGCGGTGTGTGGGCTGTAGTC
GATTTCGATGTGACCCCGTATCTGGGGAAAGCTGTTCGCTTCAATGCCACCCTGCCGGAAAATCTGCTGCAACGGATTGA
TGACGTGGTGAAGAAAGACCACCGCTATGCTTCCCGCTCTGGCTTTTTGGCCGCTGCGGCACTGCGGGAACTGTCGGTGG
CATAA