Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 19550..20075 Replicon plasmid unnamed1
Accession NZ_CP016160
Organism Klebsiella pneumoniae strain TH1 isolate TH1

Toxin (Protein)


Gene name ccdB Uniprot ID J5VBT8
Locus tag BAU11_RS25820 Protein ID WP_004197633.1
Coordinates 19550..19855 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID R4WC25
Locus tag BAU11_RS25825 Protein ID WP_015632547.1
Coordinates 19857..20075 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BAU11_RS25805 15331..16614 + 1284 WP_064081523.1 DUF1173 family protein -
BAU11_RS29095 16763..17068 + 306 WP_126034233.1 hypothetical protein -
BAU11_RS25810 17390..18175 - 786 WP_048291886.1 site-specific integrase -
BAU11_RS25815 18374..19363 - 990 WP_064081524.1 hypothetical protein -
BAU11_RS25820 19550..19855 - 306 WP_004197633.1 type II toxin-antitoxin system toxin CcdB Toxin
BAU11_RS25825 19857..20075 - 219 WP_015632547.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
BAU11_RS30255 20284..20490 - 207 Protein_23 hypothetical protein -
BAU11_RS25830 20654..21742 + 1089 WP_032638120.1 hypothetical protein -
BAU11_RS25835 21753..23126 + 1374 WP_063160997.1 hypothetical protein -
BAU11_RS25840 23467..23895 - 429 WP_023345594.1 nuclear transport factor 2 family protein -
BAU11_RS25845 24008..24853 - 846 WP_023345593.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - ybtS / ybtX / ybtQ / ybtP / ybtA / irp2 / irp1 / ybtU / ybtT / ybtE / fyuA 1..165276 165276


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11585.27 Da        Isoelectric Point: 6.4661

>T64261 WP_004197633.1 NZ_CP016160:c19855-19550 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T64261 NZ_CP016160:c19855-19550 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCTCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGGCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8362.31 Da        Isoelectric Point: 4.7247

>AT64261 WP_015632547.1 NZ_CP016160:c20075-19857 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIDMNGSFADENRDW

Download         Length: 219 bp

>AT64261 NZ_CP016160:c20075-19857 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGATATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A5MCG5

Antitoxin

Source ID Structure
AlphaFold DB A0A2L1KT86

References