Detailed information of TA system TA064212

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 3727609..3728282
• Replicon: chromosome
• Accession: NZ_CP016079
• Organism: Mesorhizobium loti NZP2037

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: A9174_RS17980
• Protein ID: WP_019859198.1
• Coordinates: 3727884..3728282 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A7R6YKV7
• Locus tag: A9174_RS17975
• Protein ID: WP_027047038.1
• Coordinates: 3727609..3727884 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9174_RS17950	3722986..3723966	+	981	WP_019859206.1	ABC transporter permease	-
A9174_RS17955	3723963..3724949	+	987	WP_019859204.1	ABC transporter permease	-
A9174_RS17960	3725066..3725614	+	549	WP_019859202.1	gluconokinase	-
A9174_RS17965	3725693..3726142	-	450	WP_019859201.1	Rrf2 family transcriptional regulator	-
A9174_RS17970	3726392..3727378	-	987	WP_019859200.1	sulfate ABC transporter substrate-binding protein	-
A9174_RS17975	3727609..3727884	+	276	WP_027047038.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
A9174_RS17980	3727884..3728282	+	399	WP_019859198.1	type II toxin-antitoxin system VapC family toxin	Toxin
A9174_RS17985	3728297..3729337	-	1041	WP_019859197.1	sulfate/molybdate ABC transporter ATP-binding protein	-
A9174_RS17990	3729407..3730291	-	885	WP_019859196.1	sulfate ABC transporter permease subunit CysW	-
A9174_RS17995	3730284..3731135	-	852	WP_019859195.1	sulfate ABC transporter permease subunit CysT	-
A9174_RS18000	3731220..3732248	-	1029	WP_019859193.1	sulfate ABC transporter substrate-binding protein	-
A9174_RS18005	3732453..3733184	-	732	WP_019859191.1	M15 family metallopeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-121)

Antitoxin

• SpoVT-AbrB domain (18-56)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14395.81 Da
• Isoelectric Point: 5.7431

>T64212 WP_019859198.1 NZ_CP016079:3727884-3728282 [Mesorhizobium loti NZP2037]
MRFMLDTNIISDMIRNPAGKAANAMVREGDAAVCTSIVVASELRYGCFRKGSTKLLKKVDDLLAEIPVLPLDVPVDVEYG
ALRAELEAVGQPIGHNDLFIAAHACVLGTTLVTANIGEFTRIRKLKVENWLE

Nucleotide

• Length: 399 bp

>T64212 NZ_CP016079:3727884-3728282 [Mesorhizobium loti NZP2037]
ATGCGGTTCATGCTGGATACCAACATCATCAGTGACATGATCCGGAACCCGGCCGGCAAGGCCGCCAATGCAATGGTGCG
CGAGGGAGACGCCGCCGTGTGCACCAGCATCGTCGTTGCCTCTGAATTGCGATATGGCTGCTTCAGGAAGGGATCGACCA
AGCTGCTCAAGAAGGTCGACGACCTGCTGGCTGAGATCCCCGTCCTGCCGCTCGACGTGCCGGTGGATGTCGAGTACGGC
GCACTTCGCGCCGAGCTGGAGGCTGTTGGCCAACCCATTGGCCACAATGACCTTTTTATTGCCGCTCACGCCTGCGTGTT
GGGCACCACGCTGGTGACGGCCAACATCGGGGAGTTCACCCGGATACGGAAGCTGAAGGTCGAGAACTGGCTGGAGTAG

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10211.63 Da
• Isoelectric Point: 4.6794

>AT64212 WP_027047038.1 NZ_CP016079:3727609-3727884 [Mesorhizobium loti NZP2037]
MPQHRSTTTPPPKEAKLFRNNRSQAVRIPVEFELPGDKVLISREGDRLVIEPLRKPGLTALLAQWAKEAPLGPEDDFPEI
NDAPVEAEDIF

Nucleotide

• Length: 276 bp

>AT64212 NZ_CP016079:3727609-3727884 [Mesorhizobium loti NZP2037]
ATGCCCCAGCATCGCAGCACAACAACGCCGCCACCGAAGGAGGCGAAACTGTTCCGCAACAACCGCAGCCAGGCGGTGCG
CATTCCTGTCGAATTCGAGCTTCCCGGCGACAAGGTTCTGATCAGCCGCGAAGGCGACCGCCTTGTCATTGAGCCGCTTC
GCAAACCTGGGCTTACCGCGCTGCTCGCCCAATGGGCGAAAGAGGCGCCGCTTGGTCCTGAGGACGACTTTCCGGAGATC
AACGATGCGCCTGTCGAAGCCGAGGACATTTTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A7R6YKV7